TABLE 1.

Microorganisms (n = 139) used to test the specificity of the TTS1 real-time PCR assay

Isolate categorySpecies, genomovar, serovar, serogroup, and/or strainaNo. tested
Genetic relatives (n = 78)Burkholderia cepacia genomovar I/III, B. multivorans, B. stabilis, B. gladioli, B. vietnamiensisT, B. cocovenenans, Burkholderia sp. strain Oklahoma35
Burkholderia mallei23
Burkholderia thailandensis5
Pandoraea apista, P. norimbergensis, P. pnomenusaT, P. sputorum8
Ralstonia paucula, R. pickettii Ralstonia sp.7
Differential diagnoses (n = 11)Acinetobacter baumanniiT1
Mycobacterium tuberculosisT1
Plasmodium falciparumT, P. malariaeT, P. ovaleT, P. vivaxT4
Staphylococcus aureus1
Vibrio cholerae2
Yersinia enterocolitica O:9, Y. enterocolitica2
Potential misidentifications (n = 6)Alcaligenes faecalis1
Chromobacterium violaceum3
Stenotrophomonas maltophilia2
Select agents (n = 6)Bacillus anthracis2
Brucella abortus, B. melitensis, B. suis3
Francisella tularensis subsp. holarctica strain LVS1
Potential sample contaminants (n = 38)Achromobacter xylosoxidans1
Aeromonas hydrophilaT1
Afipia broomeae1
Bartonella clarridgeiaeT, B. henselaeT2
Bacillus cereus3
Brucella canis1
Candida albicansT1
Enterobacter aerogenes1
Escherichia coli serotype O157:H7, E. coliT2
Haemophilus influenzae2
Klebsiella pneumoniae1
Legionella pneumophilaT1
Ochrobactrum anthropi 5D3
Proteus mirabilisT, P. vulgaris2
Pseudomonas aeruginosaT, P. mendocina, P. putida, P. stutzeri9
Psychrobacter phenylpyruvicusT1
Rothia dentocariosa1
Salmonella enterica serovar Urbana, Salmonella spp.3
Sphingobacterium multivorum1
Streptococcus pneumoniae1
  • a Superscript T indicates the type strain.