TABLE 1.

Microorganisms (n = 139) used to test the specificity of the TTS1 real-time PCR assay

Isolate categorySpecies, genomovar, serovar, serogroup, and/or strainaNo. tested
Genetic relatives (n = 78) Burkholderia cepacia genomovar I/III, B. multivorans, B. stabilis, B. gladioli, B. vietnamiensisT, B. cocovenenans, Burkholderia sp. strain Oklahoma35
Burkholderia mallei 23
Burkholderia thailandensis 5
Pandoraea apista, P. norimbergensis, P. pnomenusa T, P. sputorum8
Ralstonia paucula, R. pickettii Ralstonia sp.7
Differential diagnoses (n = 11) Acinetobacter baumannii T 1
Mycobacterium tuberculosis T 1
Plasmodium falciparum T, P. malariaeT, P. ovaleT, P. vivaxT4
Staphylococcus aureus 1
Vibrio cholerae 2
Yersinia enterocolitica O:9, Y. enterocolitica2
Potential misidentifications (n = 6) Alcaligenes faecalis 1
Chromobacterium violaceum 3
Stenotrophomonas maltophilia 2
Select agents (n = 6) Bacillus anthracis 2
Brucella abortus, B. melitensis, B. suis 3
Francisella tularensis subsp. holarctica strain LVS1
Potential sample contaminants (n = 38) Achromobacter xylosoxidans 1
Aeromonas hydrophila T 1
Afipia broomeae 1
Bartonella clarridgeiae T, B. henselaeT2
Bacillus cereus 3
Brucella canis 1
Candida albicans T 1
Enterobacter aerogenes 1
Escherichia coli serotype O157:H7, E. coliT2
Haemophilus influenzae 2
Klebsiella pneumoniae 1
Legionella pneumophila T 1
Ochrobactrum anthropi 5D3
Proteus mirabilis T, P. vulgaris2
Pseudomonas aeruginosa T, P. mendocina, P. putida, P. stutzeri9
Psychrobacter phenylpyruvicus T 1
Rothia dentocariosa 1
Salmonella enterica serovar Urbana, Salmonella spp.3
Sphingobacterium multivorum 1
Streptococcus pneumoniae 1
  • a Superscript T indicates the type strain.