TABLE 1.

Oligonucleotide sequences of primers used in AS-PCR method to determine gyrA mutation of H. pylori at positions 261, 271, and 272

Primer directionMutationPrimer namePrimer sequenceaPCR product size (bp)
ForwardC261AF261A1CCCCCATGGCGAGAAaG262
C261GF261G1CCCCCATGGCGAGAAgG262
G271AF271A5GCGATAACGCGGTTTAGaA254
F271A9GCGATAATGCGGTTTAGaA254
G271TF271T9GGCGATAATGCGGTTAATtA255
A272GF272G1GCGATAACGCGGTTTAGGgT254
F272G9GCGATAATGCGGTTTAGGgT254
ReversegyrA RGTTAGGCAGACGGCTTGGTARAATA
  • a The penultimate nucleotide to distinguish between wild-type and mutant sequences is shown in lowercase. The 1-bp mismatch at another nucleotide is underlined.