TABLE 1.

MβL-harboring clinical isolates used during the validation process

Clinical isolateMβL-encoding geneReference or accession no.Strain no.RibogroupTm of ampliconAmplicon GC content (%)
TheoreticalPractical
Serratia marcescens bla IMP-1 15 TN9106NAa77.977.538.30
Pseudomonas putida bla IMP-1 AM283489 48-12346ANA77.977.538.30
Enterobacter cloacae bla IMP-1 2a A199NA77.977.538.30
Acinetobacter baumannii bla IMP-1 AJ640197 48-696DNA77.977.538.30
P. aeruginosa bla IMP-1 19 A5386 NA77.977.538.30
Klebsiella pneumoniae bla IMP-1 11 A13309 NA77.977.538.30
P. aeruginosa bla IMP-5 19a 115-10639ANA77.576.537.23
P. aeruginosa bla IMP-13 22 86-14571NA77.176.036.17
P. aeruginosa bla IMP-16 14 101-4704164-777.376.536.70
P. aeruginosa bla IMP-16 This studyP3987164-877.376.536.70
P. aeruginosa bla IMP-18 This studyA3486NA76.476.034.57
P. aeruginosa bla VIM-1 23 75-3636CNA87.188.557.33
E. cloacae bla VIM-1 AM183120 75-10433ANA87.188.557.33
P. aeruginosa bla VIM-2 23 81-11963ANA86.988.056.81
P. aeruginosa bla VIM-2 13 49-4583CNA86.988.056.81
P. aeruginosa bla VIM-2 This studyA1254NA86.988.056.81
P. aeruginosa bla VIM-7 24 7-406NA86.287.555.00
P. aeruginosa bla SPM-1 25 48-1997A72-383.883.547.62
P. aeruginosa bla SPM-1 7 A3488 88-283.883.547.62
P. aeruginosa bla SPM-1 7 A2535 77-283.883.547.62
P. aeruginosa bla SPM-1 7 A3301 105-383.883.547.62
P. aeruginosa bla SPM-1 7 A3307 106-483.883.547.62
P. aeruginosa bla SPM-1 7 A3302 105-483.883.547.62
P. aeruginosa bla SPM-1 7 A3304 97-783.883.547.62
P. aeruginosa bla SPM-1 7 A3300 105-183.883.547.62
P. aeruginosa bla SPM-1 7 A2839 88-183.883.547.62
P. aeruginosa bla SPM-1 7 A3309 105-583.883.547.62
P. aeruginosa bla SPM-1 7 A2526 78-483.883.547.62
P. aeruginosa bla GIM-1 2 73-5671NA72.272.034.72
A. baumannii bla SIM-1 9 03-9-T104NA80.480.539.89
  • a NA, not applicable.