TABLE 2.

Comparison of sensitivities and specificities of TaqMan one-step RT-PCR assay with the in vitro transcribed RNA and spiked 25- or 50-mosquito pools

SampleQuantity (no. of copies/reaction)Avg CTa
RNase-sensitive templateRNase-resistant templateRNase-resistant template spiked 25-mosquito poolsRNase-resistant template spiked 50-mosquito pools
YFV10,000,00022.5928.8829.1829.02
1,000,00027.2933.0833.2533.08
1,00,00032.0637.4637.5937.46
10,00036.1040.5441.0441.54
1,00040.17NANDND
100NANANDND
10NANANDND
1NANANDND
JEV10,000,00019.3723.5424.1424.00
1,000,00024.9128.0028.7928.56
100,00028.0132.4633.1233.69
10,00032.1837.0137.8138.01
1,00036.7841.12NDND
10040.52NANDND
10NANANDND
1NANANDND
WNV10,000,00010.9221.7422.1422.52
1,000,00015.5526.0326.2027.00
100,00020.0130.2230.8131.26
10,00024.9634.9235.3235.98
1,00029.3739.21NDND
10037.02NANDND
10NANANDND
1NANANDND
SLEV10,000,00018.7826.1227.0027.61
1,000,00023.4430.7431.4231.74
100,00027.9735.1035.9036.10
10,00032.5439.5840.0840.02
1,00037.07NANDND
100NANANDND
10NANANDND
1NANANDND
  • a Average CT for triplicate reactions. NA, data beyond detection limit; ND, not done.