TABLE 1.

Rate of amplification of pol sequences from DBS and DPS stored at different temperatures

Panel sample identification no.Plasma RNA copies/mlPCR resulta
Plasma samplesbDBS (+RT)DBS (−RT)DPS (+RT)DPS (−RT)
Panel A (−30°C, 6 yr)
    A121,567+++
    A26,452+++
    A3338,112++++
Panel B
    B1 (room temp, 5 yr)
        B1.119,497+ND
        B1.23,230+ND
        B1.357,375+ND
    B2 (−70°C, 5 yr)
        B2.119,497++
        B2.23,230+
        B2.357,375++++
  • a RT-nested PCR were done with (+RT) or without (−RT) MuLV RT enzyme. +, positive; −, negative; ND, not done.

  • b Plasma samples were stored only at −80°C.