TABLE 1.

Effect of anti-FloR antibody on bacterial susceptibility to florfenicol and the detection of FloR protein by ELISA

E. coli strainMIC (μg · ml−1)floR geneELISA ODaFloR1 concn (ng/ml)
UntreatedAntibody treated
JM109221.147 ± 0.0091.053 ± 0.058
T/JM109221.119 ± 0.0411.238 ± 0.324
CVM1841>12832+0.330 ± 0.058113.983 ± 45.381
JM109-R6432+0.419 ± 0.02768.424 ± 11.620
C8326012864+0.287 ± 0.048145.854 ± 45.762
C83261221.166 ± 0.0130.946 ± 0.075
C832623216+0.641 ± 0.04619.196 ± 5.804
C83286328+0.557 ± 0.05231.133 ± 10.713
C83287221.100 ± 0.0661.348 ± 0.635
C83302440.973 ± 0.0282.866 ± 0.496
C83305221.147 ± 0.0341.055 ± 0.131
C8391412832+0.433 ± 0.02063.388 ± 7.718
C839166432+0.499 ± 0.04043.416 ± 11.088
  • a Absorbance at 450 nm. The mean OD reading and SD of positive antisera without FloR1 protein were 1.151 and 0.0877, respectively.