TABLE 1.

Detection of C. trachomatis diluted in UTM from FS and KS processed in AC2, AMP, and PT assaysa

Dilutions of C. trachomatis in UTM (10 tubes/dilution)No. of positive results (n = 40) obtained by NAAT:Culture in McCoy shell vial cultures (fluorescent inclusions in duplicate/ total no.)
AC2AMPPT
KSFSKSFSKSFS
10−510101010101043/40
10−610101010101010/6
10−71010710130
10−82801000
10−91400000
10−100300000
  • a Each dilution was used to inoculate 10 replicates for each swab type, resulting in a maximum of 10 positive results for each dilution-swab combination in each assay. The numbers of positive results (%) per 40 samples that were below the 100% endpoint (below 10−6) were as follows: AC2 KS, 13 (32.5%); AC2 FS, 25 (62.5%); AMP KS, 7 (17.5%); AMP FS, 11 (27.5%); PT KS, 1 (2.5%); and PT FS, 3 (7.5%). P values for these results were as follows: AC2, P < 0.001; AMP, P = 0.13; PT, P = 1.0; AC2 versus AMP, P = 0.3; AC2 versus PT, P < 0.001.