TABLE 2.

Detection of N. gonorrhoeae diluted in UTM from FS and KS processed in AC2, AMP, and PT assaysa

Dilutions of N. gonorrhoeae in UTM (10 tubes/dilution)No. of positive results (n = 30) obtained by NAAT:Culture in chocolate agar (no. of colonies/ 0.010 ml in duplicate)
AC2AMPPT
KSFSKSFSKSFS
10−4101010101010>1,000
10−510101010101048/42
10−6101046687/9
10−77902130
10−80300000
  • a Each dilution was used to inoculate 10 replicates for each swab type, resulting in a maximum of 10 positive results for each dilution-swab combination in each assay. The numbers of positive results (%) per 30 samples that were below the 100% endpoint (below 10−5) were as follows: AC2 KS, 17 (56.6%); AC2 FS, 22 (73.3%); AMP KS, 4 (13.3%); AMP FS, 8 (26.6%); PT KS, 7 (23.3%); and PT FS, 11 (36.6%). P values for these results were as follows: AC2, P = 0.06; AMP, P = 0.13; PT, P = 0.13; AC2 versus AMP, P < 0.001; AC2 versus PT, P = 0.002.