TABLE 3.

Nucleotide substitutions at the 28 nucleotide polymorphic sites in the six MVLST genes which were capable of differentiating all epidemiologically unrelated isolates analyzed in this study

IsolateSNP locations in the six virulence genes
prfAainlBinlCdalclpPlisR
****************
133233344124113433334
8265921772214764195504812683
3134038020177572345509538773
ECIbCCCGCGAGTTCCGGCTAGCAGCCGCTGC
ECII............TA.C.....T......
ECIII.T......GC.T.....T.G.T.....T
ECIV......TA....................
Boston milkb......TA..............T.....
NC soft cheeseT...T.......TA.C.....T......
PA ice cream.........C..TA.C............
IL milk....T...GC..TA.C............
United Kingdom Carlisle...A.A..AC.......T.G.T......
BL0002.T......GC..TA.C.T.G.T.A...T
BL0003....T...G...TA.C.........G.T
BL0004.T......G.....T........AT...
BL0005......TA....TA.C.....T......
BL0008.T.A....AC.......T.G.T......
BL0009.T.AT...AC.T....CT.G.T.....T
BL0010.T....TA.......C....A..AT..T
BL0011....T...GC...A.C.....T.....T
BL0012.T.AT...AC.T.....T...T......
BL0013.T.AT...AC.......T.G.T......
BL0014.T.....A.......CC......AT...
BL0015..T.T...GC..TA.C.....T......
BL0016....T...GC.....C.....T......
BL0017.T...A..AC.........G.T......
BL0018.T.A....GC.T..T..T.G.T......
BL0019.T.A.A..AC.T..T..T.G.T......
BL0020..T.T...GC...A.C.....T......
BL0021.T.............C..T....AT...
BL0022.T......G......C.......AT...
BL0023.T.....A...T..T.....A..AT...
BL0024.............A.C............
BL0025....T.......TA.C....AT......
BL0026......TA..T..........T......
BL0027......TA..T.................
BL0028.T.AT...AC.T.....T.G.T.....T
BL0029....T...G...TA.C............
BL0030....T.......TA.C..T..T.....T
BL0031.T.AA..AC.T..T..T.G.T..T..T
BL0032....T...GC..........T......
BL0033....T...GC.........G.T......
BL0034.T.A.A..GC.T..T..T.G.T..T..T
BL0035.T.A....AC.T..T..T.G.T.....T
BL0036.T.A....GC.T.....T.G.T.....T
BL0037.T.AT...AC.T.....T.G.T....CT
BL0038.T......GC.T.....T.G.T.AT..T
BL0039....T...GC...........T.....T
BL0040.T......GC.T..T..T.G.T.....T
BL0041.T.A....AC.T.....T.G.T.....T
BL0042.T.....A.......CC...A...T..T
BL0043..A....AC.T..T..T.G.T.....T
BL0044.T.A....AC.T..T..T.G.T.....T
BL0045.T.....A.......CC...A..AT..T
BL0046.T....TA.......CC...A..AT..T
BL0047..A.A..AC.T..T..T.G.T.....T
BL0048.T.A....AC.T..T..T.G.T.....T
BL0049.T.A.A..AC.T..T..T.G.T.....T
BL0050.T.A....AC.T..T..C.G.T.....T
  • a Locations of the variable nucleotide sites in the virulence genes are shown by the numbers above. Position numbers that are in boldface and start with an asterisk represent the set of 16 nucleotide substitutions capable of differentiating all epidemic clones and outbreaks. Identical nucleotides at each polymorphic site are indicated by periods.

  • b All isolates within each epidemic clone or outbreak.