TABLE 1.

Sensitivities and specificities of both assays (n = 607) utilizing two of three positives amplifications for a gold standarda

Assay and component% Positive (no. tested/total)
% Overall% Male% Female
MTRT PCR
    Sensitivity91.8 (101/110)88.8 (40/45)93.8 (61/65)
    Specificity99.5 (495/497)100 (241/241)99.2 (254/256)
    PPV98.1 (101/103)100 (40/40)96.8 (61/63)
    NPV98.2 (495/504)97.9 (241/246)98.4 (254/258)
    MG prevalence16.7 (101/607)13.9 (40/286)19.0 (61/321)
    Kappa statistic (95% CI)0.941 (0.907-0.976)0.937 (0.882-0.9992)0.944 (0.900-0.988)
TMA-MG
    Sensitivity98.1 (108/110)100.0 (45/45)96.9 (63/65)
    Specificity98.1 (488/497)97.9 (236/241)98.4 (252/256)
    PPV92.3 (108/117)90.0 (45/50)94.0 (63/67)
    NPV99.5 (488/490)100.0 (236/236)99.2 (252/254)
    MG prevalence17.7 (108/607)15.7 (45/286)19.6 (63/321)
    Kappa statistic (95% CI)0.941 (0.907-0.976)0.937 (0.882-0.9992)0.944 (0.900-0.988)
  • a Sensitivities, specificities, positive predictive values(PPVs), and negative predictive value(NPVs) are listed for both MTRT PCR and TMA-MG assays. MG prevalences for the overall population and by sex are also listed for both assays. Tests were considered positive if any two of three targets amplified. The kappa statistic measure of concordance is also listed for both assays. CI, confidence interval.