TABLE 2.

Nucleic acid sequences and locations of the primers and probes used in the enterovirus 5′ NTR RT-PCR assays

AssayPrimer or probeNucleotide sequence (5′ to 3′)Positiona
Site BForward primerGGC CCC TGA ATG CGG CTA AT449-468
Reverse primerATT GTC ACC ATA AGC AGC CA577-596
ProbeAAA CAC GGA CAC CCA AAG TAG TCG G537-561
Site CForward primerGCC CCT GAA TGC GGC TAA450-467
Reverse primerATT GTC ACC ATA AGC AGC CA577-596
ProbeAAA CAC GGA CAC CCA AAG TAG TCG538-561
ASRForward primerCCC CTG AAT GCG GCT AAT C451-469
Reverse primerAAG GAA ACA CGG ACA CCC AA546-565
Probe ATCC GCC ACA GAC TTG CGC ATT ACG A511-535
Probe BTCC GCT GCA GAG TTG CCC GTT ACG A511-535
GXEAForward primerCCC TGA ATG CGG CTA ATC C452-470
Reverse primerATT GTC ACC ATA AGC AGC CA577-596
ProbeAAA CAC GGA CAC CCA AAG TAG TCG G537-561
  • a Poliovirus genome numbering (GenBank accession no. NC_002058).