TABLE 1.

Strains used in this study and comparison of LAMP and PCR specificitya

StrainResult byb:
LAMPcIS481-PCRPTp1/p2-PCR
B. pertussis
    Tohama+++
    Yamaguchi+++
    BP256d+++
    BP257d+++
    BP258d+++
    BP259d+++
    BP260d+++
    BP289d+++
    BP290d+++
B. parapertussis
    ATCC 15237
    ATCC 15311
    BAA-587
    BPP01e+
B. hinzii
    ATCC 51730
B. holmesii
    ATCC 51541+
B. avium
    ATCC 35086
B. bronchiseptica
    R05f
  • a For PCR detections, 1 ng of DNA was tested. For LAMP assay, 10 fg DNAs of B. pertussis strains and 1 ng DNAs of other Bordetella species were tested.

  • b +, LAMP or PCR amplification; −, no LAMP or PCR amplification.

  • c Turbidity assay with a 60-min reaction.

  • d Isolated from Japanese patients in the period 2004 to 2005.

  • e Isolated from a Japanese patient in 2002.

  • f Isolated from a rabbit in 1995.