TABLE 3.

Additional oligonucleotides used for sequencinga the capsule operon and related genesb in the two Bacillus spp.

OligonucleotideSequence (5′→3′)LocationReference
acpA1RTCA-ATT-TTT-CGG-CTA-ATA-TCT-TTT-TCC-ATA-ATT68905-68937This study
acpA2RATA-ATT-TGA-AAT-TCA-TCC-CCT-ACA-ATT-CTT-AAT-G69318-69351This study
acpA4FTTT-ACA-AAG-AGG-GGG-AAA-GCA-CAA-CTT-A69747-69774This study
acpA5FTTG-CTT-ATT-ACC-GGT-GAA-GGA-AAA-AGT-TGG70103-70132This study
capA2FCGG-ATT-ATG-GTG-CTA-AGG-GAA-CTA-AAG-A55135-55162This study
RcapBCA-FGAA-ATA-GTT-ATT-GCG-ATT-GG56315-56334 23
RcapBCA-FcompCCA-ATC-GCA-ATA-ACT-ATT-TC56315-56334 23
RcapBCA-RcompCGT-ACA-GAA-GCA-GTA-GCA-CC55462-55481 23
capB1F-compCCT-TCC-TGC-TTT-TCG-ATT-TCT-TGC-TTA-TTA-AG57294-57325This study
capR2RTTT-TTG-GAG-GGA-TAC-ATA-TGG-AAC-ACA35004-35030This study
dep1RGGC-CCC-CAC-TGT-ATT-AAA-GAA-AGA-T53125-53149This study
dep2RATG-ATG-TTT-ATA-CCG-CTC-CAC-CAC-CTT53580-53606This study
dep3FACG-TAA-TTT-GGA-TCC-CCT-AGG-TTT-TTC-T53423-53450This study
dep4FTAA-GGT-TCT-CGC-TAA-ATC-TGT-CTG-GA53752-53777This study
dep4R-compTTT-CAT-ACC-TTC-CTC-AAC-CGC-AA54190-54212This study
procapRcompAGA-GGA-GTT-CTG-TAA-ATT-GCG-TTT-TTC-TT57684-57722This study
repA1FcompCGA-GTC-CCA-AAC-TTA-GCC-CTT-AAA-TCT-TC33913-33941This study
repA2FAGG-CGG-TGT-ACG-TAT-CAA-ACA-ACA-AGA-ACT33596-33625This study
repA2FcompAGT-TCT-TGT-TGT-TTG-ATA-CGT-ACA-CCG-CTT33211-33239This study
repA3FGCC-TTC-CGT-GCG-CCT-TCT-GTA33242-33265This study
repA3RGTT-AAG-CGC-TTC-GCA-CAG-TTC-TGA-T32856-32880This study
repA5FACG-TTC-CCC-GCA-GCA-CAT32840-32857This study
repA5RTTT-ACC-CAA-TTT-TAG-CAC-CAC-CAA32366-32389This study
orf61-2FTCA-GGT-ACA-TAA-TAA-AAG-CAG-AAA-GT63089-63114This study
orf61-2RGAG-AGG-AGG-GTT-GTA-AAT-GAA-GTT63826-63849This study
  • a In accordance with the manufacturer's directions, oligonucleotides were used to generate sequences that were assembled using the SeqMan program of DNAStar. In order to prevent potential PCR errors, sequences were determined multiple times in both directions.

  • b The position and orientation of each primer can be noted in Fig. 1.