TABLE 4.

Results of PCR, Southern, and dot blot hybridization for 10 ORFs and the capsule promoter of B. anthracis pX02

StrainOrganismResult for indicated ORF or capsule promoter:
ORF 38 (repA)ORF 39 (capR)ORF 43 (IS1627)ORF 48ORF 55 (capD)ORF 56 (capA)ORF 58 (capB)ORF 57 (capC)Capsule promoterORF 61ORF 64 (acpA)
63 B. anthracis + + + + + + + + + + +
118 B. circulans + + + + + + + + + + +
119 Bacillus sp. + + + + + + ±a + + + +
131 B. anthracis + + + + + + + + + + +
  • a The dot blot hybridization was negative for capB, but PCR and subsequent hybridization of Southern blots were positive. Sequencing attempts revealed the full sequence for capB in CBD 119.