TABLE 1.

Primers used for detection of hMPVa

Primer nameSequenceTarget regionNucleotide positionExpected size of fragment (bp)Reference
Published primer pairs
    MPVF-1f5′-CTTTGGACTTAATGACAGATG-3′Fusion gene3707-372444914
    MPVF-1r5′-GTCTTCCTGTGCTAACTTTG-3′4153-4134
    MPVN-3f5′-GAGAAGAGCTGGGTAGAAG-3′Nucleoprotein gene397-41538914
    MPVN-3r5′-CAAACAAACTTTCTGCT-3′786-770
    MPVF-F1-FAM5′-GAGCAAATTGAAAATCCCAGACA-3′Fusion gene3288-33103888
    MPVF-R1-FAM5′-GAAAACTGCCGCACAACATTTAG-3′3674-3652
    MPVN-F1-FAM5′-CAACAGGAAGCAAAGCAGAAAG-3′Nucleoprotein gene757-7781748
    MPVN-R15′-CAGATTCAGGACCCATTTCTC-3′951-931
    MPV-sense5′-CAAGTGTGACATTGCTGACCTGAA-3′Fusion gene3591-361411811
    MPV-antisense5′-ACTGCCGCACAACATTTAGAAA-3′3668-3652
    MPV-probe5′-TGGCYGTYAGCTTCAGTCAATTCAACAGA-3′3617-3645
“Spiked” primer
    MPVN-Stf3-f5′-GAAAAAGTRAAYACTRTATCAGAAAC-3′Nucleoprotein gene1368-1393
  • a The patient's bronchoalveolar lavage sample was tested by RT-PCR for hMPV using 5′ clinically validated primer sets. All failed, with the exception of primer set MPVN-F1-FAM/MPVN-R1 from Falsey et al. (8). Base mismatches relative to the patient's hMPV strain in the primer sequences used to detect hMPV are underlined. The nucleotide positions shown are relative to the genomic sequence of hMPV strain 00-1.