TABLE 1.

Primers used for cDNA synthesis, PCR amplification, and sequencing

PrimerSequenceAmino acid motifGeneLocationa
AN32GTYTGCCAWQTVP13009-3002
AN33GAYTGCCAWQSVP13009-3002
AN34CCRTCRTAYDGVP13111-3104
AN35RCTYTGCCAWQSVP13009-3002
224GCIATGYTIGGIACICAYRTAMLGTH(I/L/M)VP31977-1996
222CICCIGGIGGIAYRWACATM(F/Y)(I/V)PPG(A/G)VP12969-2951
292MIGCIGYIGARACNGG(Q/T)A(A/V)ETGVP12612-2627
AN89CCAGCACTGACAGCAGYNGARAYNGGbPALTA(A/V)E(I/T)GVP12602-2627
AN88TACTGGACCACCTGGNGGNAYRWACATbM(F/Y)(I/V)PPGGPVVP12977-2951
AN232CCAGCACTGACAGCAbPALTAVP12602-2616
AN233TACTGGACCACCTGGbPGGPVVP12977-2963
AN230AATTAACCCTCACTAAAGGGAGAAGATATTATACTCAYTGGcRYYTHWVP31993-2010
AN231GTCAGCTGGGTTTATNCCRTAYGINPADVP13069-3049
  • a The locations of all primers except AN230 and AN231 are those relative to the genome of PV1 Mahoney (GenBank accession number J02281); the locations for AN230 and AN231 are relative to those of the genome of EV68 Fermon (GenBank accession number AY426531).

  • b AN232 is the nondegenerate “clamp” portion of AN89, and AN233 is the nondegenerate clamp portion of AN88. Within the AN88 and AN89 sequences, these clamp regions are indicated in italic type.

  • c The T3 RNA polymerase promoter sequence is underlined.