TABLE 1.

Primers and probes used for amplification of badger IFN-γ and β-actin mRNA

DescriptionSequenceaPositionb
Badger IFNγ (F)5′-GCGCAAAGCGATAAATGAAC-3′387-406
Badger IFNγ (R)5′-CTGACTCCTTTTCCGCTTCCT-3′447-468
Badger β-actin (F)5′-GACCCTGAAGTACCCCATTGAG-3′131-152
Badger β-actin (R)5′-TTGTAGAAGGTGTGGTGCCAGAT-3′189-211
Badger IFNγ TaqMan5′-FAM-TGATGAATGATCTCTCACCAAGATCTAACC-BHQ1-3′415-445
Badger β-actin TaqMan5′-FAM-CGTCACCAACTGGGACGACATGGA-BHQ1-3′161-184
  • a FAM, 6-carboxyfluorescin; BHQ1, Black Hole Quencher 1 (nonfluorescent quencher).

  • b The position denotes the nucleotide position in the sequence with GenBank accession number Z70044 for the β-actin sequences and the sequence with GenBank accession number Y11647 for IFN-γ sequences.