TABLE 1.

GenoType MTBDRplus test results in comparison with GenoType MTBDR test results for detection of RMP and INH resistance in 75 MDR strainsa

No. (%) of strainsSequencing data, nucleotide/amino acid change(s)bMTBDR assayMTBDRplus assay
RMP pattern (rpoB)INH pattern (katG)ResultRMP pattern (rpoB)INH pattern (katG)INH pattern (inhA)Result
45 (60.0)rpoB S531L, katG 315T1cΔWT 5, Mut 3ΔWT, Mut T1MDRΔWT 8, Mut 3ΔWT, Mut T1WTMDR
2 (2.7)rpoB S531L, katG S315T1, inhA T8CΔWT 5, Mut 3ΔWT, Mut T1MDRΔWT 8, Mut 3ΔWT, Mut T1ΔWT1, Mut T8CMDR
1 (1.3)rpoB S531L, katG S315T1, inhA C15TΔWT 5, Mut 3ΔWT, Mut T1MDRΔWT 8, Mut 3ΔWT, Mut T1ΔWT1, Mut C15TMDR
2 (2.7)rpoB S531L, inhA C15TΔWT 5, Mut 3WTRMPrΔWT 8, Mut 3WTΔWT1, Mut C15TMDR
2 (2.7)rpoB S531LΔWT 5, Mut 3WTRMPrΔWT 8, Mut 3WTWTRMPr
2 (2.7)rpoB S531L, katG S315NΔWT 5, Mut 3ΔWTMDRΔWT 8, Mut 3ΔWTWTMDR
1 (1.3)rpoB S531W, katG 315T1ΔWT 5ΔWT, Mut T1MDRΔWT 8ΔWT, Mut T1WTMDR
1 (1.3)rpoB S531W, inhA C15TΔWT 5WTRMPrΔWT 8WTΔWT1, Mut C15TMDR
1 (1.3)rpoB S531WΔWT 5WTRMPrΔWT 8WTWTRMPr
1 (1.3)rpoB S531P, katG S315T1ΔWT 5ΔWT, Mut T1MDRΔWT 8ΔWT, Mut T1WTMDR
1 (1.3)rpoB S531L, katG S315T1, T2ΔWT 5, Mut 3ΔWT, Mut T1, T2MDRΔWT 8, Mut 3ΔWT, Mut T1, Mut T2WTMDR
1 (1.3)rpoB H526D, katG 315T1ΔWT 4, Mut 2BΔWT, Mut T1MDRΔWT 7, Mut 2BΔWT, Mut T1WTMDR
2 (2.7)rpoB H526L, katG 315T1ΔWT 4ΔWT, Mut T1MDRΔWT 7ΔWT, Mut T1WTMDR
2 (2.7)rpoB H526N, katG 315T1ΔWT 4ΔWT, Mut T1MDRΔWT 7ΔWT, Mut T1WTMDR
1 (1.3)rpoB H526N, katG S315T1, inhA C15TΔWT 4ΔWT, Mut T1MDRΔWT 7ΔWT, Mut T1ΔWT1, Mut C15TMDR
2 (2.7)rpoB H526R, katG S315T1, inhA C15TΔWT 4ΔWT, Mut T1MDRΔWT 7ΔWT, Mut T1ΔWT1, Mut C15TMDR
1 (1.3)rpoB H526R, ahpC C52TΔWT 4WTRMPrΔWT 7WTWTRMPr
1 (1.3)rpoB D516V, katG 315T1ΔWT 2, Mut 1ΔWT, Mut T1MDRΔWT 3, 4, Mut 1ΔWT, Mut T1WTMDR
1 (1.3)rpoB D516V, katG S315T1, inhA C15TΔWT 2, Mut 1ΔWT, Mut T1MDRΔWT 3, 4, Mut 1ΔWT, Mut T1ΔWT1, Mut C15TMDR
1 (1.3)rpoB N518I, katG S315T1ΔWT 2, Mut 1ΔWT, Mut T1MDRΔWT 3, 4, Mut 1ΔWT, Mut T1WTMDR
1 (1.3)rpoB del514-516, katG S315T1ΔWT 1, 2ΔWT, Mut T1MDRΔWT 2-4ΔWT, Mut T1WTMDR
1 (1.3)rpoB S522Q, katG 315T1ΔWT 3ΔWT, Mut T1MDRΔWT 5, 6ΔWT, Mut T1WTMDR
1 (1.3)rpoB Q513PΔWT 1WTRMPrΔWT 2, 3WTWTRMPr
1 (1.3)rpoB V176F, katG K152T, ahpC G48AWTWTSusc.WTWTWTSusc.
  • a Resistance was determined by conventional DST. r, resistant; Susc., susceptible; Δ deletion; WT, wild type; Mut, mutation.

  • b According to previously published data (28); the GenBank accession numbers are L27989 for the rpoB gene, X68081 for the katG gene, U66801 for the inhA gene, and U16243 for the ahpC-oxyR intergenic region.

  • c S315T1, AGC→ACC/Ser→Thr exchange; S315T2, AGC→ACA/Ser→Thr.