TABLE 2.

Primer sequences and characteristics of housekeeping gene loci included in the S. mutans MLST scheme

LocusGene locus tag and putative function of gene productsPrimer namesPrimer sequences (5′ to 3′)Size of sequence fragment (bp)No. of alleles identifiedNo. (%) of polymorphic nucleotidesdN/dSGenBank accession no.
tkt Smu.291, transketolasetktFACC CGG GTG TTG TCA TGG GCG CTG C
tktRCAT AGG ATG ACG CTT CGC CAG AAA C4321416 (3.70)0.1032 AB281702-AB281802
glnA Smu.364, glutamineglnA-FCCT TGG GGA GAT GAA AAC GGA GCC G
    synthetaseglnA-RTGG CCA TAA AGG TTG CAT ACA AAC C4621615 (3.24)0.0628 AB281803-AB281903
gltA Smu.365, glutamategltA-FTGC CTT AAC GAT GTT AGA GAG AAT G
    synthasegltA-RAAA GAC TAT CTT CAA AAG CAC ACC C3872321 (5.43)0.2082 AB281904-AB282004
glk Smu.542, glucoseglk-FGAC AAG TTC AGG AGA AAT GGG CTA T
    kinaseglk-RCAG CAA CTC CAT GAA TAA GAT TGC C4021916 (3.98)0.6333 AB282005-AB282105
aroE Smu.778, ShikimatearoE-FGAT GAA GTA ACG AAA GCA GCA GAT T
    5-dehydrogenasearoE-RTGC CAT CCA TAC CAA CAT TGG TCG C3962119 (4.80)0.4467 AB282207-AB282307
gyrA Smu.1114, DNA gyrasegyrA-FTAC AGG TGA TGT CAT GGG TAA ATA C
    subunit AgyrA-RCCG GGT AGT ACT TCC ATT AGG TCA C4321717 (3.94)0.1520 AB282106-AB282206
murI Smu.1718, glutamatemurI-FTCC GGA GTG GGC GGT TTA ACG GTC G
    racemasemurI-RTCA ACA ATA GGA ACA AAT TTG GGG C4231517 (4.02)0.0544 AB282308-AB282408
lepC Smu.1874, signallepC-FCCG CGT CTC TTT ATC TGG TTT CTT G
    peptidase IlepC-RGAC AAT GCG ATC ATC ACC TAA AAG C4172319 (4.56)0.1305 AB282409-AB282509