TABLE 2.

Antifungal susceptibilities, genotypes, and expression characteristics of CgCDR1 and CgCDR2 genes for sequential C. glabrata isolates from each of 15 patients

Patient no.Isolate no.Isolation dayaMIC (μg/ml) forb:Genotype by:Fold gene expression fore:
AmBFluItraVoriEKREAG-NcMLSTdCgCDR1CgCDR2
11-1011610.25K1N1ST72.30.4
1-22811610.5K2N1ST72.01.6
22-10180.1250.125K3N2aST34.00.8
2-21180.1250.125K4N2aST32.91.9
2-32180.1250.125K5N2aST33.21.2
33-10180.250.06K6N3aST34.43.6
3-21180.1250.03K7N3bST31.81.2
44-10180.1250.25K8N2bST35.14.2
4-21180.250.125K8N2cST34.32.4
4-34180.250.06K9N2dST37.36.8
4-46180.250.06K10N2dST35.74.0
4-57180.250.06K8N2dST34.44.1
55-100.53210.5K11N4aNew type3.50.6
5-2113210.5K11N4bNew type4.11.7
66-1013210.25K12N5aST77.84.3
6-2113210.25K13N5bST712.46.7
77-100.51610.25K14N6aST711.01.0
7-220.51610.25K14N6aST76.50.6
7-3f360.512842K14N6aST7145.03.5
88-100.5160.50.25K15N7aST75.62.8
8-211160.250.125K15N7aST73.32.6
8-3f80.5160.250.25K15N7bST73.63.2
8-4f220.53210.5K15N7cST719.94.1
99-1013210.5K16N8aST73.12.5
9-2113220.5K16N8bST73.13.8
9-3f2313220.5K16N8aST73.74.4
1010-1013210.5K16N8aST72.63.3
10-2f413210.5K16N8aST73.14.4
10-3f813220.5K16N8bST73.11.6
1111-1f00.25160.50.25K17N9ST32.23.2
11-2f10.25160.50.25K17N9ST33.76.7
1212-100.5160.50.25K18N10aST71.12.0
12-240.5320.50.25K18N10aST70.42.0
12-3711610.25K18N10bST70.30.9
1313-100.51610.25K19N1ST72.33.8
13-2111610.25K19N1ST72.75.6
1414-100.580.50.25K20N6bST73.71.0
14-2120.580.50.25K21N6bST75.13.5
14-3f240.580.50.25K22N6bST75.91.4
1515-101160.50.06K23N11aST310.03.7
15-2f111160.50.06K23N11bST312.24.9
  • a The numbers indicate the days after the first positive blood culture in each patient.

  • b AmB, amphotericin B; Flu, fluconazole; Itra, itraconazole; Vori, voriconazole.

  • c A minor change (subtypes, a, b, c, and d) in REAG-N patterns for serial isolates from the same patient suggests microevolution.

  • d STs were assigned by combining the data from each of the six loci (FKS, LEU2, NMT1, TRP1, UGP1, and URA3) sequenced per isolate, as described in reference 9. The allele profiles for the six loci were 578736 for ST3, 344334 for ST7, and 365239 for the new ST.

  • e Quantification was performed using real-time RT-PCR. The values are averages of three independent experiments and represent increases in the levels of gene expression relative to that of C. glabrata ATCC 90030 (set as 1.0).

  • f Isolate was recovered after the initiation of antifungal therapy.