TABLE 2.

Detailed comparison of microscopy, nested PCR, and LAMP for malaria parasite detection and species identification

Parasite(s) detected by each method (no. of samples)a
MicroscopyNested PCRLAMPb
P. falciparum (12)c P. falciparum (12) P. falciparum (12)
P. falciparum + P. vivax (5) P. falciparum + P. vivax (4), P. vivax (1) P. falciparum + P. vivax (4), P. vivax (1)
P. vivax (34) P. vivax (30), P. vivax (1), P. vivax (1), P. ovale (2) P. vivax (30), P. vivax + P. falciparum (1), negative (1), P. ovale (2)
P. malariae (12) P. malariae (8), P. malariae + P. vivax (1), P. ovale (1), P. malariae (1), negative (1) P. malariae (8), P. malariae + P. vivax (1), P. ovale (1), Plasmodium spp.d (1), P. malariae (1)
P. ovale (5) P. ovale (5) P. ovale (5)
Negative (53)Negative (50), P. falciparum (1), P. vivax (2)Negative (50), P. falciparum (1), P. vivax (2)
  • a Results that were nonconcordant between nested PCR and LAMP are underlined.

  • b LAMP assays were run twice; in all cases the two experiments gave the same results.

  • c Each row provides the results obtained with identical DNA samples.

  • d Positive for the genus Plasmodium by LAMP but negative by all four species-specific LAMPs.