TABLE 2.

Comparison between detection of PUUV IgM and IgG antibodies and PUUV RNA in serum samples from patients with suspected NE

Sample no.Day of sampling after disease outbreakPUUV IgMaPUUV IgG titerPUUV RNAbPUUV RNAc (copies/ml)
15+<40+107,125
2Unknown+320+171
34+160+12,255
42+80+56,620
54+>640+40,965
64+>640+37,365
71+>640+11,741
88+80+5,485
95+320+4,113
10Unknown+>640+1,543
115+40+/−3,599
127+160+74,645
13Unknown+640+2,999
145+<40+12,684
153+40+24,167
168+640+2,828
1716+160+75,587
184+80+9,429
19Unknown+>640NDd1,371
203+80+177,485
217+<40+70,274
22Unknown+160+71,302
23Unknown+<40+2,914
24Unknown+>640+14,912
258+320+18,854
261+<40+120,494
273+80+55,705
285+320ND18,425
297+<40ND29,138
30Unknown+320ND14,569
31Unknown+>640ND1,542
324+320+3,000,000
337+<40ND450,000
348+320+54,000
356+>640+950
36Unknown+320+100,000
374+>640+240,000
387+>640-0
39Unknown+<40ND0
40Unknown+160ND0
41Unknown+>640ND0
422+>6400
43Unknown+3200
44Unknown+3200
45Unknown+/−40+244
464+/−<40ND1,800,000
47Unknown+/−160+74,000
4817+/−3200
49Unknown+/−3200
508+/−>6400
515+/−<400
524<40+251,958
53-1001-21<40ND0
  • a IgM was diluted 1:16 and scored as negative (−), positive (+), or weakly positive (+/−) by IF.

  • b Detected by RT-PCR. After two-step RT-PCR, the amplification product was detected by agarose gel electrophoresis and scored as negative (−) or positive (+).

  • c Detected by real-time RT-PCR.

  • d ND, not determined.