TABLE 4.

Allele-specific real-time PCR cycle times for known STsa

Gene + polymorphb CT for ST:
13093
arcC210GCF 19.30 28.2631.62
arcC210GTJ31.53 18.99 23.29
arcC210GAFUndet32.4236.44
tpi241 + 243ATGJUndet21.9325.35
tpi241 + 243GTGF26.84 16.97 20.72
tpi241 + 243GTAJ 19.11 26.6030.13
arcC162CTF 18.44 28.28 19.07
arcC162CAJ28.35 17.73 31.46
gmk318AAJ 23.78 21.724.51
gmk318ATFUndet 20.96 20.37
tpi36TJ30.4521.92 20.93
tpi36CF 19.65 18.3 24.95
pta294AAF 20.75 UndetUndet
pta294ACJ24.38 20.07 18.88
pta294ATF36.2719.6521.41
pta383CTJ 23.04 21.9221.98
pta383CAF32.47 19.82 21.38
  • a Boldface type indicate reactions with perfectly matched allele-specific primers. Undet, undetermined.

  • b 5′ bases and subterminal mismatches are indicated. F, FAM labeling; J, JOE labeling.