TABLE 1.

Origin, methicillin phenotype, and typing results for 59 S. aureus isolatesf

IsolateCountry/yrSource (hospital designation)PFGE typeaMLVF typebTriplex PCR typeSTAR-RP (AluI/Tru9I) typecMLST typeaspa typeaMethicillin phenotype (S. aureus type)d
C115ePoland/1994AA1111 (1/1)45t015HeMRSA
2688/98ePoland/1998BA2211 (1/1)45t015HeMRSA
A005aePoland/1992CA4321 (1/1)45t390MSSA
MR89ePoland/1992DB1432 (2/2)239t037HoMRSA
3121ePoland/1996EB3532 (2/2)239t388HoMRSA
MR11Poland/1992DB7632 (2/2)239t037HoMRSA
MR1064ePoland/1992FB8743 (3/3)239t037HoMRSA
N98ePoland/1995CB13853 (3/3)239t037HoMRSA
A005bePoland/1994GB28933 (3/3)239t037HoMRSA
H390ePoland/1994HB451032 (2/2)239t388HoMRSA
1791/97ePoland/1997IC11163 (3/3)157t030HoMRSA
1794/97Poland/1997IC21164 (4/4)157t030HoMRSA
1807/97Poland/1997IC41164 (4/4)157t030HoMRSA
2689ePoland/1998BC71272 (2/2)239t037HoMRSA
MR1003ePoland/1992JC81163 (3/3)239t030HoMRSA
N104aePoland/1995GC101133 (3/3)157t030HoMRSA
3028ePoland/1996KD11382 (2/2)247t052HoMRSA
2233/97ePoland/1997LD21382 (2/2)336t052HoMRSA
2234b/97Poland/1998LD21382 (2/2)336t052HoMRSA
2255/98Poland/1998LD21382 (2/2)336t052HoMRSA
2258/98Poland/1998LD21382 (2/2)336t052HoMRSA
2577/98Poland/1998LD21382 (2/2)336t052HoMRSA
303/00ePoland/2000MD121382 (2/2)247t052HoMRSA
2260/98Poland/1998LD131382 (2/2)247t052HoMRSA
BN4ePoland/1996KE1495 (5/5)1t159HeMRSA
MR80ePoland/1992DF11516 (3/6)5t053HeMRSA
2700ePoland/1998BF21516 (3/6)5t053HeMRSA
MR52Poland/1992DF31516 (3/6)5t053HeMRSA
MR5Poland/1992DF51616 (3/6)5t053HeMRSA
MR84Poland/1992DF61616 (3/6)5t053HeMRSA
MR29Poland/1992DF71516 (3/6)5t053HeMRSA
MR31Poland/1992DF71516 (3/6)5t053HeMRSA
MR24Poland/1992DF81716 (3/6)5t053HeMRSA
N39ePoland/1995KF818106 (3/6)5t389HeMRSA
MR44Poland/1992DF101916 (3/6)5t053HeMRSA
MR63Poland/1992DF101616 (3/6)5t053HeMRSA
MR27Poland/1992DF131516 (3/6)5t053HeMRSA
1899/96ePoland/1996EG120112 (2/2)80t044HeMRSA
2838/98Poland/1996EG221112 (2/2)80t044HeMRSA
MR1010aePoland/1992DG322112 (2/2)80t044HeMRSA
B098ePoland/1994NJ23123 (3/3)239t037HoMRSA
771ePoland/1997OK22432 (2/2)239t037HoMRSA
2690ePoland/1998BK32544 (4/4)239t387HoMRSA
MR76ePoland/1992DK526133 (3/3)239t037HoMRSA
J405ePoland/1994PK142433 (3/3)239t037HoMRSA
MR47Poland/1992DK1627142 (2/2)239t037HoMRSA
2956ePoland/2001QN228157 (6/7)30t391HeMRSA
EMRSA-16eEngland/1992RN329168 (7/8)36t018HeMRSA
2684ePoland/1998KN530178 (7/8)30t019MSSA
3301eSlovenia/1998SO131182 (2/2)501t037HoMRSA
3497eBulgaria/1998TO232192 (2/2)239t037HoMRSA
3254eTurkey/1996UP33203 (3/3)239t030HoMRSA
3498eRussia/1998VQ34219 (8/9)8t008HoMRSA
3502eBulgaria/1998WR35222 (2/2)1t386HeMRSA
3248eCzech Republic/1996XS36238 (7/8)30t012HoMRSA
3521eLithuania/1998YT37219 (8/9)8t008HoMRSA
NCTC 8325eU38243 (3/3)8t211
794ePoland/1997OW39252 (2/2)15t084MSSA
3483eSlovenia/1998ZY40266 (3/6)228t001HeMRSA
  • a Results previously obtained by Malachowa et al. (11).

  • b The same results were produced by both multilocus VNTR typing methods, MLVF (11) and expanded MLVF (this study).

  • c Restriction profiles obtained using restriction endonucleases AluI and Tru9I.

  • d Abbreviations for methicillin phenotypes: MSSA, methicillin-susceptible S. aureus; HeMRSA, S. aureus heterogeneously resistant to methicillin; HoMRSA, S. aureus homogeneously resistant to methicillin.

  • e Epidemiologically unrelated isolates used for calculation of the discriminatory power.

  • f Numbers of patterns obtained for all isolates (total; n = 59) and for epidemiologically unrelated isolates (total; n = 40) are as follows: for PFGE, 52 and 40, respectively; for MLVF, 40 and 34; for triplex PCR, 26 and 25; for STAR-RP, 9 (8/9) and 9 (8/9); for MLST, 14 and 14; and for spa typing, 20 and 20.