TABLE 1.

16S species identification for routine isolates

No. of strainsGroup studieda16SCommercial system(s)Species identification (%)cReference
Size(s) (bp)DatabasebCriteria (%)cConvComm16S
72GNB1,189, 527, 418MicroSeqCMConv, MIDI, Biolog9067.7-84.689.216
328Mycobacteria500MicroSeq≥99Conv.4262.58
83GNB, GPB527MicroSeqCMVitek 2, Phoenix77.11006
231Bacteroides899, 711GenBank≥99Conv74.583.114
47CNS1,500GenBank>97API StaphID, Phoenix63.8-85.187.29
20GPA1,500GenBank≥98Vitek ANA, RapID ANA II,20-456510
MicroSeq    API 20A
107GNNFB796GenBank, EMBL, DDBJ≥99API 20NE, Vitek 253.2-54.291.62
  • a CNS, coagulase-negative staphylococci; GNB, gram-negative bacteria; GNNFB, gram-negative nonfermentative bacteria; GPA, gram-positive anaerobes; GPB, gram-positive bacteria.

  • b DDBJ, DNA Data Bank Japan; EMBL, European Molecular Biology Laboratory.

  • c CM, closest match; Comm, commercial system; Conv, conventional phenotypic tests.