TABLE 4.

List of isolates that produced ambiguous species identification or that were not identified by the RLB assay

StrainSpecies received asSpecies identification by:
RLB with ITS1 probeRLB with ITS2 probeITS sequencing (% identity with GenBank sequences)
Reference strains
    CBS 5149 Candida haemulonii C. haemulonii + C. norvegensis C. haemulonii + C. norvegensis C. haemulonii (100)b
    WM 889 C. haemulonii C. haemulonii + C. norvegensis C. haemulonii + C. norvegensis C. haemulonii (100)b
    WM 890 C. haemulonii C. haemulonii + C. norvegensis C. haemulonii + C. norvegensis C. haemulonii (100)b
    WM 891 C. haemulonii C. haemulonii + C. norvegensis C. haemulonii + C. norvegensis C. haemulonii (100)b
Clinical isolates
    AS7 Aspergillus niger NSa A. niger A. niger c
    AS31 A. niger NS A. niger A. niger c
    AS33 A. niger NS A. niger A. niger c
    AS36 A. niger NS A. niger A. niger c
    AS37 A. niger NS A. niger A. niger c
    AS39 A. niger NS A. niger A. niger c
  • a Abbreviation: NS, hybridization with fungus-specific probes but not with species-specific probes.

  • b Sequence analysis yielded sequences with 100% identity to C. haemulonii sequences in GenBank (accession nos. AY50035 and AJ606467 ).

  • c Sequence analysis yielded sequences with 100% identity to A. niger ATCC 16888 sequence (GenBank accession no. AY373852 ) but with a single base polymorphism (from A to G) with the GenBank sequence used to design the probe (GenBank accession no. AY213633 ).