TABLE 3.

Evaluation of the real-time PCR assays with DNA extracted from blood specimensa

Clinical diagnosis and blood specimenPatient informationMean CT value (no. of CT values used) for:
Clinical outcomeType of positive cultureAssay 8653Assay 9438Assay for orf11
Septicemia by Burkholderia pseudomallei
    BP1SurvivedB32.23 (5)32.56 (2)30.21 (2)
    BP2SurvivedB32.68 (5)34.28 (7)32.13 (2)
    BP3SurvivedB/P/TS33.80 (5)33.54 (6)31.28 (2)
    BP4DiedB/TS/U30.87 (5)33.73 (4)33.53 (2)
    BP5DiedB/S/TS36.03 (6)37.48 (4)37.35 (2)
    BP6SurvivedB/P32.92 (3)34.16 (7)NA
    BP7DiedB/P/S/TS/U25.32 (5)24.35 (6)22.71 (2)
    BP8SurvivedP34.60 (4)38.74 (4)Neg
    BP9SurvivedP34.77 (3)36.77 (3)38.23 (1)
    BP10DiedB/P/S/U32.50 (5)34.06 (4)NA
    BP11SurvivedB/P/S/U33.49 (4)37.48 (4)Neg
    BP12SurvivedB/P34.64 (5)36.90 (7)37.07 (2)
    BP13SurvivedP/TS37.80 (5)36.63 (2)39.12 (2)
    BP14DiedB32.30 (9)33.67 (6)35.10 (2)
    BP15SurvivedB35.59 (6)34.61 (2)42.27 (1)
    BP16SurvivedB33.64 (2)NegNA
    BP17SurvivedB33.66 (3)Neg32.96 (2)
    BP18SurvivedP35.41 (3)NegNeg
    BP19DiedB/S34.62 (9)Neg37.53 (2)
    BP20DiedB/TS33.02 (2)NegNeg
    BP21SurvivedBNegNegNeg
    BP22SurvivedB/TSNegNegNeg
    BP23SurvivedPNegNegNeg
    BP24SurvivedS/TSNegNegNeg
    BP25SurvivedB/PNegNegNeg
    BP26SurvivedBNegNegNeg
    BP27SurvivedPNegNegNeg
    BP28SurvivedB/P/UNegNegNeg
Septicemia by Burkholderia cepacia
    CI1DiedB/SNegNegNeg
Septicemia by Escherichia coli
    CI2SurvivedBNegNegNeg
    CI3SurvivedBNegNegNeg
    CI4SurvivedB/UNegNegNeg
    CI5SurvivedB33.56 (6)33.83 (5)39.31 (2)
Septicemia by Enterobacter sp.
    CI6SurvivedBNegNegNeg
Septicemia by Klebsiella pneumoniae
    CI7SurvivedB29.92 (4)30.03 (6)33.3 (2)
    CI8DiedB/SNegNegNeg
    CI9SurvivedB/S/UNegNegNeg
    CI10SurvivedBNegNegNeg
    CI11SurvivedBNegNegNeg
Septicemia by Staphylococcus aureus
    CI12DiedB/P/SNegNegNeg
    CI13SurvivedB36.27 (5)NegNeg
    CI14SurvivedB/UNegNegNeg
Septicemia by Streptococcus sp.
    CI15DiedBNegNegNeg
    CI16SurvivedB/CSFNegNegNeg
    CI17DiedBNegNegNeg
  • a Evaluation of the real-time PCR assays was performed with DNA extracted from blood specimens collected from 45 septicemic patients admitted to various hospitals in the northeastern region, Thailand. Abbreviations: B, blood; P, pus; S, sputum; TS, throat swab; U, urine culture; CSF, cerebrospinal fluid culture; Neg, negative amplifications; NA, not enough DNA samples were left for performing these real-time PCRs.