TABLE 1.

Chinese VanA-type vancomycin-resistant E. faecium clinical isolatesa

StrainHospital (city)Date of isolation (yr/mo/day)SourceDiagnosis/underlying diseasebAntibiotic(s) usedMLST resultcesp statusdTn1546-like elementTransfer frequency of vancomycin resistancef (per donor cell)MIC (μg/ml)
Sequence type (nearest)Clonal complexAllelic profile
atpAddlgdhpurKgydpstSadkCTypeeLocationBroth mating (4 h)Filter mating (16 h)VANTECAMPGENSTRTETERYCHLRIFLVXLZD
I125E (Dalian)2001/12/9SputumCachexia/gastric cancerCEPs, CLIST78CC7815111111NVIPlasmid (pI125Vg)<10−71.6 × 10−451216128>1,02420480.551284162
C264A (Beijing)2003/8/21BileCholangitis/hepatocirrhosisVAN, CEPsST320* (ST80)CC117911112291NVPlasmid (pC264Vg)<10−72.3 × 10−725632128>1,024640.5512324322
ZB11A (Beijing)2004/1/15UrinePyelitis/gastric cancerVANST335* (ST172)CCS45*1334*15192918*NIIPlasmid<10−73.0 × 10−55122560.25>1,024320.55121651212
ZB14B (Beijing)2004/2/13UrinePyelitis/leukemiaCEPsST321* (ST31)CC2807311113NIIPlasmid<10−73.7 × 10−6512256128>1,024>4,0960.255128442
ZB15B (Beijing)2004/2/27UrinePyelitis/kidney dysfunctionTECST78CC7815111111NIIPlasmid<10−71.4 × 10−3512256128>1,024320.25512164642
ZB16B (Beijing)2004/4/4UrinePyelitis/hepatocirrhosisCEPsST18CC187111511NIIPlasmid<10−75.7 × 10−6512256128>1,02416851284642
ZB18B (Beijing)2004/4/9BloodSepsis/leukemiaVAN, CEPsST25CC259316111NIPlasmid (pZB18)3.1 × 10−47.0 × 10−15122562162,04825625664<0.122
ZB19C (Beijing)2005/10/18SputumPneumonia/pulmonary dysfunctionVAN, CEPsST322* (ST262)CC187161571PIIPlasmid<10−71.7 × 10−5512512128>1,024320.255121681282
ZB20C (Beijing)2005/10/20UrinePyelitis/rectal cancerVAN, CEPsST203CC78151111201PIIIPlasmid<10−71.8 × 10−525664128>1,0246464512168322
ZB21D (Tianjin)2005/10/12BloodSepsis/hepatocellular cancerVAN, MEMST78CC7815111111PIVPlasmid<10−72.8 × 10−651216128128320.25512164642
ZB22B (Beijing)2005/10/28SputumPneumonia/COPDVAN, MEMST323* (ST17)CC175111111PIIPlasmid (pZB22)4.2 × 10−78.5 × 10−3512512128>1,024326451244642
ZB23C (Beijing)2005/11/7SputumPneumonia/bronchiectasisCEPsST203CC78151111201PIIPlasmid<10−72.7 × 10−6512256128>1,024320.551216<0.1642
ZB24C (Beijing)2005/11/8UrinePyelitis/COPDCEPsST203CC78151111201PIIPlasmid<10−73.1 × 10−6512256128>1,024320.2551216<0.1642
  • a Drug abbreviations: CEPs, broad-spectrum cephalosporins; CLI, clindamycin; VAN, vancomycin; TEC, teicoplanin; MEM, meropenem; AMP, ampicillin; GEN, gentamicin; STR, streptomycin; TET, tetracycline; ERY, erythromycin; CHL, chloramphenicol; RIF, rifampin; LVX, levofloxacin; LZD, linezolid.

  • b COPD, chronic obstructive pulmonary disease.

  • c *, new ST/allele in this study.

  • d The esp gene was detected by PCR amplification, as described in the text. P, positive; N, negative.

  • e Tn1546-like elements were typed by DNA sequence structure, as shown in Fig. 4.

  • f The wild-type strain and E. faecium BM4105RF were used as donor and recipient, respectively (13).

  • g Plasmids were identified in our previous report (35).