TABLE 1.

A. Genotypic and phenotypic characteristics of clumping factor- or coagulase-deficient S. aureus strains

Strain groupaPFGE type (nb)PFGE subtype (nb)GenotypePhenotype (nb)Clone characteristics
STSCCmec typePresence of clfA, clfB, coa, spa, and nuc genes (nb)Clonal complexagr groupMethicillin resistance phenotypeGlycopeptide resistance phenotypecDelta-hemolysin productiondRelatedness to Polish and international S. aureus clones (reference[s])eCenter codefYr or period of isolation
1A (1)A1 (1)45IV+ (4)CC45IMRSAGSSA+HeMRSA-Pol4 (23), Berlin clone (36)352001
A (3)A4 (2), A13 (1)45CC45IMSSAGSSA+7, 191999-2004
B (34)B5 (16), B8, B46, B47, B48 (3), B49, B50 (4), B51, B52, B53, B54, B55, B56, B57239, 241III, IIIB, IIIC+ (34)CC8IMRSAGSSA; hGISA (2)+ (32); − (2)HoMRSA-Pol1 (23), Hungarian clone (9)1, 3, 6, 14, 19, 20, 22, 25, 26, 27, 31 40, 411997-2003
C (2)C4239III+ (2)CC8IMRSAGSSA+He/HoMRSA-Pol1 (23), Brazilian clone (2)11997
K (2)K17, K18 (2)239, 254+ (5)CC8IMSSAGSSA+26, 411998-2001
K (3)K19, K21239IIIC+ (3)CC8IMRSAGSSA+HoMRSA-Pol3 (23)5, 251999, 2003
R (3)R115+ (3)CC15IIMSSAGSSA+Polish MSSA clone15, 23, 371996, 1999, 2002
N (1)N930+CC30IIIMSSAGSSA+HeMRSA-Pol3 (23), SWP4 clone (36, 49)101997
F (1)F145IV+CC5IIMRSAGSSA+HeMRSA-Pol1 (23), pediatric MRSA clone (23, 33)352001
G (1)G180IV+SingletonIIIMRSAGSSA+HeMRSA-Pol2 (23), Mediterranean clone (1, 17)71996
RR (1)RR1121+CC51IVMSSAGSSA+161999
Y (1)Y2395+SingletonIMSSAGSSA+Sporadic351999
ZZ (1)ZZ1 (1)7+SingletonIMSSAGSSA+Sporadic42002
2D (30)D1, D6 (3), D7 (23), D27, D30 D32247IA+ (30)CC8IMRSAhGISA (23); GSSA− (22), + (8)HoMRSA-Pol2 (23), Iberian clone (33)4, 5, 8, 9,10, 11, 12, 13, 15, 19, 21, 24, 28, 29, 30, 31, 32, 33, 34, 351996-2004
N (1)N9 (1)30+CC30IIIMSSAGSSA+HeMRSA-Pol3 (23), SWP4 clone (36, 49)361998
R (3)R1, R2, R315+ (3)CC15IIMSSAGSSA+Polish MSSA clone38, 352002, 2000 1996
K (1)K23239III+CC8IMRSAhTISAHoMRSA-Pol3 (23)21998
WW(1)XX1707+SingletonIIIMSSAGSSA+Sporadic231996
YY (1)YY1182+SingletonIMSSAGSSA+Sporadic101997
3A (1)A1 (1)45IA+CC45IMRSAGSSA+HoMRSA-Pol4 (23), Berlin clone (36)38
D (3)D30, D7 (2)247IA+CC8IMRSAhTISA (1), GSSA (1)− (1), + (1)HoMRSA-Pol2 (23), Iberian clone (33)5, 31
R (3)R115+ (3)CC15IIMSSAGSSA+Polish MSSA clone13, 7, 18
N (3)N8, N10, N1130+CC30IIIMSSAGSSA+HeMRSA-Pol3 (23), SWP clone (36, 49)16, 38
XX (3)XX11+CC1IIMSSAGSSA+7, 35, 27
T (1)T11+CC1IIIMSSAGSSA+35
K (1)K22239+CC8IMSSAGSSA+17
RR (1)RR2121+CC51IVMSSAGSSA+Sporadic35
NY (1)NY15I+CC5IIMRSAGSSA+EMRSA-3 (36)16
Y (1)Y1395+SingletonIIMSSAGSSA+Sporadic12
X (1)X2446+SingletonIIIMSSAGSSA+Sporadic7
  • a Group 1, based on phenotypical tests: CF(+), COA(−), n = 54; NUC(−), n = 32; NUC(+), n = 22; MAN(−), n = 1; MAN(+), n = 53. Group 2, based on phenotypical tests: CF(−), COA(+), n = 37; NUC(−) n = 1; NUC(+), n = 36; MAN(−), n = 1; MAN(+), n = 36. Group 3, based on phenotypical tests: CF(+), delayed coagulase, COA(+), n = 19; NUC(−), n = 10; NUC(+), n = 10; MAN(−), n = 0; MAN(+), n = 19.

  • b n, number of isolates.

  • c The PAP method was used to confirm the hGISA/hTISA phenotype.

  • d +, enhanced zone of hemolysis created by the interaction of beta-hemolysin of ATCC 25923 and delta-hemolysin of the test strain; −, lack of enhanced zone of hemolysis (lack of delta-hemolysin production).

  • e HeMRSA and HoMRSA are Polish clones. SWP, Southwest Pacific.

  • f Center code number reflects the locations of Polish cities or foreign countries of origin of isolates, listed here in alphabetical order: 1, Bielsko Biala; 2, Bulgarian; 3, Bydgoszcz; 4, Bytom; 5, Czestochowa; 6, Gdansk; 7, Grajewo; 8, Hungary; 9, Kalisz; 10, Konin; 11, Koszalin; 12, Krakow1; 13, Krakow2; 14, Lomza; 15, Makow Maz.; 16, Mlawa; 17, Nowa Sol; 18, Olsztyn; 19, Otwock; 20, Poznan; 21, Rzeszow; 22, Siemianowice Sl.; 23, Sieradz; 24, Slovenia; 25, Sochaczew; 26, Suwalki; 27, Szczecin; 28, Warsaw, Waw1; 29, Waw2; 30, Waw3; 31, Waw4; 32, Waw5; 33, Waw6; 34, Waw7; 35, Waw8; 36, Waw9; 37, Wejherowo; 38, Wolomin; 39, Zabrze; 40, Zamosc; 41, Zielona Gora.