TABLE 5.

ORFs in and around 108PVL and their similarities to three extant PVL-carrying phages

ORF or special structureaStarting positionEnding positionSize (bp)aabGeneFunctionData for phage
φPVLφSLTφSa2mw
% Identityc to genomeCorresponding ORF (bp)% aa identity% Identity to genomeCorresponding ORF (bp)% aa identity% Identity to genomeCorresponding ORF (bp)% aa identity
(P001)8034026187Hypothetical protein
attB124627025Attachment site on chromosome
Core27129929Core sequence
attP left30032425Attachment site on phage
P00215883831,206402intPhage integrase98.6Integrase (1,206)98.698.4Integrase (1,206)98.499.9Integrase (1,206)99.9
P0032094232122876Putative membrane protein54.651.1100Putative membrane protein MW1441 (624)100
P00429162521396132Hypothetical protein49.199.7phiSLTp03 (396)99.799.5MW1439 (396)99.5
P00533792945435145Putative lipoprotein50.399.5phiSLTp04 (435)99.599.5Putative lipoprotein MW1438 (435)99.5
P00641503434717239clcl-like repressor81.4Repressor (771)81.446.8Repressor (315)48.749.5Repressor (324)45.2
P0074314455624381croPutative cro-like repressor49.853.351.2Putative cro-like repressor (249)54.5
P00845725324753251antPutative antirepressor84.3Antirepressor (750)84.345.7Antirepressor (549)47.645No corresponding gene
P00953375753417139Transcriptional regulator97.9orf32 (276)98.44847.8
P0105830606924080Hypothetical protein48.750.449.8
P01163466023324108Hypothetical protein48.449.446.9
P01263966722327109Hypothetical protein52.947.552.2
P0136755693718361Hypothetical protein4952.655.9
P0146967723026488Putative DNA binding protein99.6orf37 (264)99.695.1phiSLTp12 (264)95.182.3MW1431 (264)81.7
P0157498777928294Hypothetical protein50.775.6phiSLTp14 (303)75.648
P0167780806428595Hypothetical protein95.7orf39 (261)98.199.3phiSLTp15 (261)99.248.8
P01780798558480160phiPV8348.151.348.3
P01885589331774258Hypothetical protein46.84746.7
P01993619912552184ssbSingle-strand binding protein47.9Ssb (471)49.948.8Ssb (444)50.147.6
P020992510596672224Hypothetical protein47.470.1phiSLTp19 (675)70.146.2
P0211155910702858286Hypothetical protein46.447.449.5
P0221162412394771257Phage replication protein48.180phiSLTp21 (771)8045.3
P0231240413183780260DNA replication protein48.397.4phiSLTp22 (780)97.452.1
P02413240133389933Hypothetical protein55.261.658.2
P025133511357222274Hypothetical protein50.796.8phiSLTp23 (222)96.847.8
P0261358313987405135Hypothetical protein50.468.351
P0271417814549372124Hypothetical protein85.7orf50 (369)85.986.5phiSLTp26 (369)86.757.2
P028145501479824983Hypothetical protein92.3orf51 (243)93.893.8phiSLTp27 (243)93.890.4MW1420 (243)92.1
P0291480715178372124Hypothetical protein5051.250.5
P030151711541924983Hypothetical protein97.2orf52 (249)97.294.4phiSLTp28 (249)94.451.2
P0311539415939546182dutdUTPasc87.2dUTPase (528)87.2100dUTPase (528)10047.4
P032162371647323779Hypothetical protein48.7100phiSLTp32 (237)10048.1
P033166151681520167Hypothetical protein99.5orf60 (204)99.562.648.1
P0341683817290453151Hypothetical protein100orf61 (471)10051.847
P0351740517857453151Hypothetical protein99.6orf62 (453)99.648.349.4
P0361785718216360120Phage endonuclease100orf63 (342)10049.750.6
P0371834618813468156terSPhage terminase, small subunit100Terminase (468)10049.1Terminase (306)47.948.9Terminase (306)47.6