TABLE 2.

Dot blot hybridization screening of 121 NT H. influenzae isolates from the middle ears of patients with otitis media, 137 NT H. influenzae isolates from the throats of healthy children, and 39 H. influenzae type b isolates probed with 12 sPCR fragments

sPCR probeInsert size (bp)Isolates from throats of healthy children (n = 137)Isolates from the middle ears of patients with otitis media (n = 121)H. influenzae type b isolates (n = 39)
No. of isolates% with sPCR fragmentsNo. of isolates% with sPCR fragmentsPRP valueNo. of isolates% with sPCR fragmentsPRP value
sJPX1406664029.27763.6a2.18<0.0001000<0.0001
sJPX1321,1717655.510788.41.59<0.0001391001.80<0.0001
sJPX1202615036.56553.71.470.0055391002.74<0.0001
sJPX12410319367.911595.01.40<0.0001391001.47<0.0001
sJPX16361510073.012099.21.36<0.00013897.41.330.0011
sJPX8497410173.712099.21.35<0.0001391001.360.0003
sJPX16176111986.912099.21.140.0002391001.150.0169
sJPX17642711986.91211001.15<0.00013897.41.120.0604
sJPX14759312389.811998.41.100.0045391001.110.0375
sJPX10132312692.01211001.090.0014391001.090.0676
sJPX1706445439.45545.51.150.337112.60.06<0.0001
sJPX5888812692.010990.10.980.5953391001.090.0676
  • a Boldface indicates statistically significant differences (P < 0.05) in the distribution of probe hybridization compared to that for the isolates from the throats of healthy children.