TABLE 3.

Comparison of sequence data and LiPA results: overview of the results obtained for the six codonsa

CodonNo. (%) of samplesPartial concordanceNo. (%) of samples
Full concordanceFull concordancePartial discordanceComplete discordanceIND on LiPATotal
WTMUTMIXNo. (%) of samplesNo. of samples MIX on LiPA/ no. of samples WT or MUT on sequencing
80170 (87.6)1605521 (10.8)12/91 (0.5)b2c194
173188 (96.9)18712 (1.0)1/14c194
180181 (93.3)176512 (6.2)8/41c194
181172 (88.7)168421 (10.8)21/01c194
204162 (83.5)14217332 (16.5)26/6194
236185 (95.4)1857 (3.6)7/01 (0.5)b1d194
    Total1,058 (90.9)1,018231795 (8.2)75/202 (0.2)9 (0.8)1,164
  • a WT, wild type; MUT, mutant; MIX, mixture; IND, indeterminate.

  • b A mutation was also detected by LiPA at an earlier or later time point.

  • c Indeterminate results were due to weak reactivities (always in samples with viral loads less than 1,000 copies/ml).

  • d Indeterminate result due to a polymorphism in the probe region.