TABLE 3.

Biological features and incidence of a typical phenotype of nine species of Malassezia

Species identified by molecular biological analysisNo. of strainsColony characteristic on CHROM (% of incidence)aGrowth characteristic (% of incidence)bCatalase reaction
SizeColor/morphologyPrecipitateSDATE slantEL slant40°C
M. pachydermatis43Large (100)Pale pink/smooth (100)+ (100)Growth (95.3)dGrowth and produced a black zone (95.3)dGrowth (95.3)dNT+ (100)
M. sympodialis84Large (100)Pale pink/smooth (100)+ (100)No growth (100)Growth and produced a black zone (100)No growth (100)NT+ (100)
M. globosa14Small (100)Purple/smooth (100)+ (100)No growth (100)No growth and no change (100)No growth (100)NT+ (100)
M. dermatis5Large (100)Pale pink to purple/smooth (100)+ (100)No growth (100)Growth and no change (100)No growth (100)NT+ (100)
M. furfur41Large (100)Pale pink/wrinkled (100)− (97.6)cNo growth (100)Growth and produced a black zone (100)Growth (85.4)NT+ (100)
M. slooffiae108Small (100)Pale pink/smooth (100)− (100)No growth (100)Growth and no change (65.7)eNo growth (98.1)Growth (84.3)e+ (100)
M. obtusa4Medium (100)Pink/rough (100)− (100)No growth (100)No growth but produced a black zone (100)No growth (100)NT+ (100)
M. restricta71Small (100)Pink/smooth (100)− (100)No growth (100)No growth and no change (100)No growth (100)NT− (100)
M. japonica2Large (100)Pink/smooth (100)− (100)No growth (100)Growth and produced a black zone (100)No growth (100)No growth (100)+ (100)
  • a Incubated at 32°C for 4 to 7 days.

  • b Incubated at 32°C for 4 days. NT, not tested; +, positive; −, negative.

  • c Only one strain of fresh clinical isolate produced a precipitate.

  • d Only two strains of fresh clinical isolates did not grow on SDA and EL and did not produce a black zone on TE.

  • e Thirty-seven strains of fresh clinical isolates produced a black zone on TE, and 17 strains did not grow on modified Leeming and Notman agar at 40°C for 4 days.