TABLE 3.

The spa complex of MRSA isolates as determined by visual analysis

MLST sequence typespa complex (n)aspa typeSSR profileNo. of IsolatesOther characteristicsGenetic backgroundb (n)a
ST5 (1-4-1-4-12-1-10)cspa class A (41)t00226-23-17-34-17-20-17-12-17-1615CA (13/81, 16.0%)A (41)
t60126-23-17-34-34-17-20-17-12-17-1613HA (28/57, 49.1%)
t2458d26-16-34-34-17-20-17-12-17-168agr group II, hlg-2
t2460d26-17-34-34-17-20-17-17-17-163
t01026-17-34-17-20-17-12-17-161
t30626-23-17-34-17-20-17-12-17-17-161
ST72 (1-4-1-8-4-4-3)spa class B (33)t32407-23-12-12-17-20-17-12-12-1725CA (22, 27.2%),B (33)
t66407-23-12-12-17-20-17-12-174HA (11, 19.3%)
t14807-23-12-21-12-17-20-17-12-12-172agr group I, hlg-2
t90107-23-12-17-20-17-12-12-171
t2461d07-23-12-12-12-17-20-17-12-12-171
ST239 (2-3-1-1-4-4-3)spa class C (29)t03715-12-16-02-25-17-2427CA (15, 18.5%)C (27)e
ST580 (3-35-48-19-20-26-39)t02115-12-16-02-16-02-25-17-241HA (14, 24.6%)
ST30 (2-2-2-2-6-3-2)t13808-16-02-25-17-241hlg-2, agr group I (1)
ST1 (1-1-1-1-1-1-1)spa class D (24)t28607-23-13-34-16-34-33-1321CA (22, 27.2%)D (24)
ST493 (62-1-1-1-1-1-1)t153307-23-13-13-16-34-33-131HA (2, 3.5%)
ST573 (1-1-1-1-12-1-1)t2457d07-23-13-34-34-16-34-33-131agr group III, hlg-2
t2459d07-23-34-34-16-34-33-131
ST89 (1-26-28-18-18-33-50)spa class E (8)t37549-13-23-05-17-34-33-347CA (8, 9.9%)E (8)
t172849-20-13-23-05-17-34-33-341agr group III, hlg
ST8 (3-3-1-1-4-4-3)Singletont00811-19-12-21-17-34-24-34-22-251ST8 (PVL, agr group I)
ST188 (3-1-1-8-1-1-1)Singletont18907-23-12-21-17-341ST188 (agr group I)
  • a Defined by visual analysis as described by Ruppitsch et al. (28); 137 of 138 isolates were analyzed, as 1 HA isolate was not amplified by spa PCR.

  • b Determined by MLST profile with one or two allelic variants corresponding to the spa complex.

  • c Includes the ST5 single locus variant.

  • d Newly reported in this study.

  • e ST580 and ST30 were excluded because of an MLST profile with three or more allelic variants.