TABLE 1.

Evaluation of the multiplex PCR respiratory assay with temporally different strains of influenza virus and HRSV

Virus (subtype)aGenotypeaYrTypeStrainTm (°C)
Influenza virus A (H1)1999-2000Clinical85.10
1999-2000Clinical85.39
2000-2001Clinical85.12
2000-2001Clinical85.20
2000-2001Clinical85.04
2001-2002Clinical84.62
2001-2002Clinical85.07
2002-2003Clinical85.50
2002-2003Clinical85.29
1995VaccineA/Beijing/262/9585.06
1933VaccineA/WSN/3385.25
    Mean (H1)85.15 ± 0.22
Influenza virus A (H3)1998-1999Clinical85.58
1998-1999Clinical85.18
1998-1999Clinical85.35
1998-1999Clinical85.57
1998-1999Clinical85.37
2001-2002Clinical85.07
2001-2002Clinical85.65
2002-2003Clinical84.92
1999VaccineA/Panama/2007/9985.18
1997VaccineA/Sydney/5/9785.06
    Mean (H3)85.31 ± 0.25
    Mean (influenza virus A)85.27 ± 0.22
Influenza virus B1998-1999Clinical83.87
1998-1999Clinical83.12
2000-2001Clinical83.01
2000-2001Clinical83.03
2000-2001Clinical82.88
2000-2001Clinical83.75
2000-2001Clinical83.17
2002-2003Clinical83.89
2002-2003Clinical83.84
1997VaccineB/Harbin/7/9784.12
    Mean (influenza virus B)83.47 ± 0.46
HRSVB1999-2000Clinical79.32
A1999-2000Clinical79.83
A2000-2001Clinical79.06
A2000-2001Clinical78.85
B2001-2002Clinical79.34
B2001-2002Clinical79.52
B2001-2002Clinical79.98
B2001-2002Clinical79.52
A2001-2002Clinical79.84
B2001-2002Clinical79.42
B2001-2002Clinical79.49
B2001-2002Clinical79.48
B2001-2002Clinical79.55
B2001-2002Clinical79.32
A2001-2002Clinical79.77
A2001-2002Clinical79.92
A2001-2002Clinical79.40
    Mean (HRSV-A)79.52 ± 0.43
    Mean (HRSV-B)79.49 ± 0.19
    Mean (all HRSV)79.51 ± 0.30
  • a Subtyping was performed by multiplex PCR for the influenza virus hemagglutinin gene (2), whereas genotyping was performed by conventional PCR for the HRSV G gene (22).