TABLE 2.

Antisera used in this study

AnimalImmunizing antigen or animal statusDilutiona
Mouse 47M. avium subsp. paratuberculosis K-10 sonicate1:3,000
Mouse 48M. avium subsp. paratuberculosis K-10 sonicate1:3,000
Rabbit 270Live M. avium subsp. paratuberculosis1:1,000
Rabbit 275Heat-killed M. avium subsp. paratuberculosis1:1,500
Rabbit 273Heat-killed M. avium subsp. paratuberculosis1:1,000
Rabbit 274Heat-killed M. avium subsp. paratuberculosis1:1,000
Cow 67Naturally infected clinical cow1:500
Cow 598Naturally infected clinical cow1:500
Cow 159Naturally infected clinical cow1:500
Cow 167Naturally infected clinical cow1:500
Cow 14Naturally infected clinical cow1:500
Cow 144Naturally infected clinical cow1:500
Cow 47Naturally infected clinical cow1:500
Cow KayNaturally infected clinical cow1:500
Cow 116Naturally infected clinical cow1:500
Cow 200Naturally infected clinical cow1:500
Cow 107Control cow1:500
Cow 108Control cow1:500
Cow 109Control cow1:500
Cow 110Control cow1:500
Cow 111Control cow1:500
Cow 181Control cow1:500
Cow 182Control cow1:500
Cow 183Control cow1:500
Cow 184Control cow1:500
α-MBPMonoclonal antibody developed at NADC1:3,000
  • a Working dilution used in immunoblotting experiments.