TABLE 1.

Primers used to amplify and clone M. avium subsp. paratuberculosis-specific sequences

Gene no.aVectorPrimerbSequencec
10pMal c-2ForATCCTCTAGACGGCGGATCAGCATCTAC
RevGCGCAAGCTTCACCTCATCGTGGCCAGGTT
pCRT7/CTForATGCGCCCGCACACCGGCGGACGG
RevCCTCATCGTGGCCAGGTTGTGCCA
11pMal c-2ForATCCTCTAGAACCGAACACGAGTGGAGCA
RevGCGCAAGCTTCAGACTCTGACCGACGTCAT
pCRT7/CTForATGGTGGCAACCGAACACGAG
RevTCAGACTCTGACCGACGTCAT
38pMal c-2ForATCCTCTAGAAGCATTTCGGCTCCCACGGTG
RevGCGCAAGCTTCTACGTCGGTTCGGCGCGCAT
pCRT7/CTForATGGTGGTAAGCATTTCGGCTC
RevCGTCGGTTCGGCGCGCATCAAAGC
56pMal c-2ForATCCTCTAGAATGAACACTTCTTCCTCTCTA
RevGCGCAAGCTTCATATCGCGGTGATCCTGAC
pCRT7/CTForATGAACACTTCTTCCTCTCTA
RevTATCGCGGTGATCCTGACCGC
57pMal c-2ForATCCTCTAGAATGGCCACCAACGACGACCA
RevGCGCAAGCTTCACGCGGCCGTCGGGCCGGCTG
pCRT7/CTForATGGCCACCAACGACGACCAA
RevCGCGCGGCCGTCGGGCCGGC
135pMal c-2ForATCCTCTAGACGCATCGGCGCGCGCGAGCAG
RevGCGCAAGCTTCATAGGACCTCCCGCGCTGC
pCRT7/CTForATGGCGGGGATGCCGGAGGAG
RevTAGGACCTCCCGCGCTGCGCT
159pMal c-2ForATCCTCTAGAATGCGTTTCGCCCTCCCG
RevGCGCAAGCTTCACGCCTTGATTTCGTCCTG
pCRT7/CTForATGCGTTTCGCCCTCCCGACGCGC
RevCGCCTTGATTTCGTCCTGCCGCGA
217pMal c-2ForATCCTCTAGAATGGCCGAACGCGGACTGTTC
RevGCGCAAGCTTCTAGGAATCCGCGTCGACGAT
pCRT7/CTForATGCGCAGGCTGAGTGCCGAAC
RevGGAATCCGCGTCGACGATCTT
218-cpMal c-2ForCCTCTTCTAGACGCATGCGATCGAACAGT
RevGCGCAAGCTTCACGAGTTGCGGCGGGCGAC
218-npMal c-2ForAGGCTGTCTAGAACGCTGCGG
RevGCGCAAGCTTCACAGCGGCCCGACCCCGGC
218 (full length)pCRT7/CTForATGCGCGGAAACCGCAGCGAGTTC
RevACGTTGCAGGCAGGCCGAGCACAG
219pMal c-2ForATCCTCTAGAACATCTACTGAGCGCCGTTTG
RevGCGCAAGCTTCACGCCGCCACCCCGTCCCG
pCRT7/CTForGTGTCAACATCTACTGAGCGC
RevCGCCGCCACCCCGTCCCGGCG
228pMal c-2ForATCCTCTAGAGTGCCATACGCCGAATCG
RevGCGCAAGCTTCTAGGAGCCTGCTGAATT
pCRT7/CTForATGCCATACGCCGAATCGCCCAGG
RevGGAGCCTGCTGAATTAATTCCGCG
240pMal c-2ForATCCTCTAGAGCTGCCTTGTGGAGCATCCG
RevGCGCAAGCTTCTAGCGCTCACGAAAGTAAGC
pCRT7/CTForATGGTCATTGCGCTAGCTGCCTTG
RevGCGTCCACGAAAGTAAGCCACT
241pMal c-2ForCCTCTTCTAGACCGTCGCTGAAGCTCACCGTCATC
RevGCGCAAGCTTCACAGAGGTCGATCGGGTCG
pCRT7/CTForATGCCGTCGCTGAAGCTCACC
RevCAGAGGTCGATCGGGTCGAGG
250pMal c-2ForATCCTCTAGAGGCTCCTCGATGCTAACCGCT
RevGCGCAAGCTTCAGTCGGCCGGCGAAACGCC
pCRT7/CTForATGGTTGCGGCGCAAGGCTCCTCG
RevGTCGGCCGGCGAAACGCCCGCGGC
251pMal c-2ForATCCTCTAGAATGGTGCGCGACGGCAGCCGC-
RevGCGCAAGCTTCACGTGCTGTCCCCATCGGC
pCRT7/CTForATGGTGCGCGACGGCAGCCGCCGC
RevCGTGCTGTCCCCATCGGCGTCCTC
252pMal c-2ForATCCTCTAGAATGACCACCGACAACCCCACG
RevGCGCAAGCTTCATGAGGGCTGTCCCTC
pCRT7/CTForATGACCACCGACAACCCCACGCCC
RevTGAGGGCTGTCCCTCTCCGGCGGC
253pMal c-2ForATCCTCTAGATTGACCGCGTTGACGGCGTTG
RevGCGCAAGCTTCAGCGGTCCGCGCTCTTCGC
pCRT7/CTForGCGGTCCGCGCTCTTCGCATCGGC
RevTTGACCGCGTTGACGGCGTTGCGC
254pMal c-2ForATCCTCTAGACGCCAGCGCTACAAGGTGATT
RevGCGCAAGCTTCACGCGCTCCTTTCAGCCTT
pCRT7/CTForATGAGCGACGAGTTACGCCAGCG
RevCGCGCTCCTTTCAGCCTTGCGGCG
255pMal c-2ForATCCTCTAGACTTGCGCGGCTGGTGGGGGTT
RevGCGCAAGCTTCAGTCACCCCGCGGCCGGTA
pCRT7/CTForATGGTCAACGTGCCGCGTGCG
RevGTCACCCCGCGGCCGGTAGTC
256pMal c-2ForATCCTCTAGAATGGCATACCGAATGAGT
RevGCGCAAGCTTCATGACCCTGCCGGCGTCCC
pCRT7/CTForATGGCATACCGAATGAGTCCC
RevTGACCCTGCCGGCGTCCCGGA
257pMal c-2ForATCCTCTAGAGTGCCAGTCGGCACGACA
RevGCGCAAGCTTCAACTGACCACCAGGGCCGG
pCRT7/CTForGTGCCAGTCGGCACGACACC
RevACTGACCACCAGGGCCGGCCG
  • a Gene numbers are consistent with those presented in reference 2.

  • b For, forward; Rev, reverse.

  • c Listed 5′ to 3′.