TABLE 1.

Probes and primers used in the study

TargetOrganismStrainaPrimer or probeSequencebOligonucleotide concn (pmol/μl)
ITS Bartonella spp.Bart/16-23F5′-bio-TTGATAAGCGTGAGGTCGGAGG0.4
Bart/16-23R5′-bio-CAAAGCAGGTGCTCTCCCAG0.4
B. henselae Bh-Sp1S-HENS5′-a-ATCGGTTCAATCATATCGCTTT3.2
B. bacilliformis CIP 77.27S-BACI5′-a-CCTATGATTGATTTCTAGGC0.4
B. bovis CIP 106292S-BOV25′-a-CGTTTTGATAGTCTTTTGTGTTGC0.4
B. koehlerae CIP 107025S-KOE5′-a-TTAAATTATATCACTTTGGGTCATACG0.4
B. vinsonii subsp. berkhoffiiCIP 104960S-VIN-B5′-a-TTTCGGACACTATTGATAAA3.2
B. vinsonii subsp. arupensisCIP 106848S-VIN-A15′-a-ACTTGTTGGAATTGCTTAACC3.2
B. vinsonii subsp. vinsoniiCIP 103738S-VIN-A25′-a-ATGAAAATATTGAGAGATTTG3.2
B. taylori CIP 107028S-TAY5′-a-TATCCATTTCGCTTAGGCA3.2
B. doshiae CIP 107026S-DOSH5′-a-TTTGAACCTTCTCTCTTTAT3.2
B. grahamii CIP 107024S-GRAH25′-a-ATTCAAGTTGATGAATTTGGTTAT3.2
B. elizabethae CIP 103761S-ELIZ5′-a-TAAGTTCCCTTCAAGAGGATA3.2
B. tribocorum CIP 105476S-TRIB5′-a-TTCTATTAAGTTTGTCAAAGGG0.4
B. clarridgeiae CIP 104772S-CLAR5′-a-ACGATGCTAAAAGTTGCTAT3.2
B. alsatica CIP 105477S-ALS5′-a-GCTGGTGAAACTTGCTTATA3.2
B. quintana CIP 107027S-QUIN5′-a-CGCTTATCCATTTGGTTTAA3.2
B. chomelii CIP 107869S-CHOSCA5′-a-TTATGATTGCTGATAAGTTTGCTG0.4
B. schoenbuchensis CIP 107819
B. capreoli CIP 106691
B. birtlesii CIP 106294
Bartonella strain from T. europaeaT4cS-TALP5′-a-CAGTCCCTTTAGGTCCATTTAATC3.2
16S rRNA Bartonella spp.16S-R5′-bio-GCCYCCTTGCGGTTAGCACAGCA1
P24Emod5′-bio-CCTTCAGTTMGGCTGGATC1
S-BART16S5′-a-CTCGCCCTTAGTTGCCAGCATT3.2
  • a Strains from the Pasteur Collection used for Fig. 1, except B. henselae Bh-Sp1, which was isolated from blood of a cat and belongs to the Centro Nacional Microbiología collection.

  • b bio, biotin modification; a, amino link modification.

  • c No isolate; sample where this Bartonella strain was detected.