TABLE 2.

eap-targeting oligonucleotide primers used in this study

NameOligonucleotide sequence (5′→3′)Amplicon size (bp)Source or reference
EAP-CON1TAC TAA CGA AGC ATC TGC C230This study
EAP-CON2TTA AAT CGA TAT CAC TAA TAC CTC
EAP-P3wtGCA GCT AAG CCA TTA GAT AAA TCA TCA AGT TGC TTA CAC CVariableaDerived from primer P3 (19)b
EAP-P2wtTTA AAA TTT AAT TTC AAT GTC TAC TTT TTT AAT GTC
  • a Strain-specific amplicon size depending on the number of tandem repeats in the eap gene.

  • b The primer sequences were modified by omitting the originally introduced restriction sites.