TABLE 2.

The effects of temperature crossover and nitrate and pyruvate supplementation on culture of stressed Aspergillus hyphaea

Approximate no. of hyphal fragmentsbCulture condition
35°C35°C × 24 h, then 25°C25°C × 24 h, then 35°C25°CSupplement at 25°Cc
10524/2424/2424/2424/2424/24
10424/2424/2424/2424/2424/24
10324/2424/2423/2420/2423/24
1026/244/246/243/246/24
1015/242/240/240/241/24
1003/240/240/240/240/24
  • a Growth versus no growth is shown as a fraction of 24 inoculation spots on SDAE plates for each dilution and condition after 14 days of incubation.

  • b The initial inoculum contained approximately 107 hyphal fragments per ml.

  • c Supplementation with 10 mM NaNO3 and 10 mM potassium pyruvate.