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Journal of Clinical Microbiology
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antimicrobial resistance

  • Open Access
    Reflex Detection of Ciprofloxacin Resistance in <span class="named-content genus-species" id="named-content-1">Neisseria gonorrhoeae</span> by Use of the SpeeDx ResistancePlus GC Assay
    Bacteriology
    Reflex Detection of Ciprofloxacin Resistance in Neisseria gonorrhoeae by Use of the SpeeDx ResistancePlus GC Assay

    Resistance-guided therapy (RGT) for gonorrhoea may reduce unnecessary use of broad-spectrum antibiotics. When reflexed from the Aptima Combo 2 assay, the ResistancePlus GC assay demonstrated 94.8% sensitivity and 100.0% specificity for Neisseria gonorrhoeae detection.

    Darren Y. J. Lee, Melinda M. Ashcroft, Eric P. F. Chow, Michelle Sait, Vesna De Petra, Marlene Tschaepe, Sigrid Lange, George Taiaroa, Catriona S. Bradshaw, David M. Whiley, Christopher K. Fairley, Benjamin P. Howden, Marcus Y. Chen, Shivani Pasricha, Deborah A. Williamson
  • Comparing BioFire FilmArray BCID2 and BCID Panels for Direct Detection of Bacterial Pathogens and Antimicrobial Resistance Genes from Positive Blood Cultures
    Letter to the Editor
    Comparing BioFire FilmArray BCID2 and BCID Panels for Direct Detection of Bacterial Pathogens and Antimicrobial Resistance Genes from Positive Blood Cultures
    Venere Cortazzo, Tiziana D’Inzeo, Liliana Giordano, Giulia Menchinelli, Flora Marzia Liotti, Barbara Fiori, Flavio De Maio, Francesco Luzzaro, Maurizio Sanguinetti, Brunella Posteraro, Teresa Spanu
  • Open Access
    Genomic Serotyping, Clinical Manifestations, and Antimicrobial Resistance of Nontyphoidal <em>Salmonella</em> Gastroenteritis in Hospitalized Children in Ho Chi Minh City, Vietnam
    Bacteriology
    Genomic Serotyping, Clinical Manifestations, and Antimicrobial Resistance of Nontyphoidal Salmonella Gastroenteritis in Hospitalized Children in Ho Chi Minh City, Vietnam

    Nontyphoidal Salmonella (NTS) are among the most common etiological agents of diarrheal diseases worldwide and have become the most commonly detected bacterial pathogen in children hospitalized with diarrhea in Vietnam. Aiming to better understand the epidemiology, serovar distribution, antimicrobial resistance (AMR), and clinical manifestation of NTS gastroenteritis in Vietnam, we conducted a clinical genomics investigation of...

    Vu Thuy Duong, Hao Chung The, Tran Do Hoang Nhu, Ha Thanh Tuyen, James I. Campbell, Pham Van Minh, Hoang Le Phuc, Tran Thi Hong Chau, Nguyen Minh Ngoc, Lu Lan Vi, Alison E. Mather, Stephen Baker
  • Rapid Detection of Azole-Resistant <span class="named-content genus-species" id="named-content-1">Aspergillus fumigatus</span> in Clinical and Environmental Isolates by Use of a Lab-on-a-Chip Diagnostic System
    Mycology
    Rapid Detection of Azole-Resistant Aspergillus fumigatus in Clinical and Environmental Isolates by Use of a Lab-on-a-Chip Diagnostic System

    Aspergillus fumigatus has widely evolved resistance to the most commonly used class of antifungal chemicals, the azoles. Current methods for identifying azole resistance are time-consuming and depend on specialized laboratories. There is an urgent need for rapid detection of these emerging pathogens at point-of-care to provide the appropriate treatment in the clinic...

    Ling-Shan Yu, Jesus Rodriguez-Manzano, Nicolas Moser, Ahmad Moniri, Kenny Malpartida-Cardenas, Nicholas Miscourides, Thomas Sewell, Tatiana Kochina, Amelie Brackin, Johanna Rhodes, Alison H. Holmes, Matthew C. Fisher, Pantelis Georgiou
  • Evaluation of the SpeeDx MG <em>parC</em> (Beta) PCR Assay for Rapid Detection of <span class="named-content genus-species" id="named-content-1">Mycoplasma genitalium</span> Quinolone Resistance-Associated Mutations
    Letter to the Editor
    Evaluation of the SpeeDx MG parC (Beta) PCR Assay for Rapid Detection of Mycoplasma genitalium Quinolone Resistance-Associated Mutations
    Emma L. Sweeney, Kym Lowry, Samantha Ebeyan, Marie Lundgren, David M. Whiley
  • Comparative Genomics and Antimicrobial Resistance Profiling of <em>Elizabethkingia</em> Isolates Reveal Nosocomial Transmission and <em>In Vitro</em> Susceptibility to Fluoroquinolones, Tetracyclines, and Trimethoprim-Sulfamethoxazole
    Bacteriology
    Comparative Genomics and Antimicrobial Resistance Profiling of Elizabethkingia Isolates Reveal Nosocomial Transmission and In Vitro Susceptibility to Fluoroquinolones, Tetracyclines, and Trimethoprim-Sulfamethoxazole

    The Elizabethkingia genus has gained global attention in recent years as containing sporadic, worldwide, nosocomial pathogens. Elizabethkingia spp. are intrinsically multidrug resistant, primarily infect immunocompromised individuals, and are associated with high mortality (∼20 to 40%). As yet, gaps remain in our understanding of transmission, global strain relatedness, antimicrobial resistance, and effective therapy....

    Delaney Burnard, Letitia Gore, Andrew Henderson, Ama Ranasinghe, Haakon Bergh, Kyra Cottrell, Derek S. Sarovich, Erin P. Price, David L. Paterson, Patrick N. A. Harris
  • Prevalence of <span class="named-content genus-species" id="named-content-1">Mycoplasma genitalium</span> and Antibiotic Resistance-Associated Mutations in Patients at a Sexually Transmitted Infection Clinic in Iceland, and Comparison of the S-DiaMGTV and Aptima <span class="named-content genus-species" id="named-content-2">Mycoplasma genitalium</span> Assays for Diagnosis
    Bacteriology
    Prevalence of Mycoplasma genitalium and Antibiotic Resistance-Associated Mutations in Patients at a Sexually Transmitted Infection Clinic in Iceland, and Comparison of the S-DiaMGTV and Aptima Mycoplasma genitalium Assays for Diagnosis

    Mycoplasma genitalium is prevalent among attendees in sexually transmitted infection (STI) clinics, and therapy is hampered by rapidly rising levels of resistance to azithromycin and moxifloxacin. In this study, we evaluated, for the first time in Iceland, the prevalence of M. genitalium and...

    Ingibjörg Hilmarsdóttir, Eva Mjöll Arnardóttir, Elísabet Reykdal Jóhannesdóttir, Freyja Valsdóttir, Daniel Golparian, Ronza Hadad, Hannes Bjarki Vigfússon, Magnus Unemo
  • Genotyping of <span class="named-content genus-species" id="named-content-1">Mycoplasma genitalium</span> Suggests <em>De Novo</em> Acquisition of Antimicrobial Resistance in Queensland, Australia
    Letter to the Editor
    Genotyping of Mycoplasma genitalium Suggests De Novo Acquisition of Antimicrobial Resistance in Queensland, Australia
    Emma L. Sweeney, Jacob Tickner, Cheryl Bletchly, Graeme R. Nimmo, David M. Whiley
  • Open Access
    Species Identification and Antibiotic Resistance Prediction by Analysis of Whole-Genome Sequence Data by Use of ARESdb: an Analysis of Isolates from the Unyvero Lower Respiratory Tract Infection Trial
    Epidemiology
    Species Identification and Antibiotic Resistance Prediction by Analysis of Whole-Genome Sequence Data by Use of ARESdb: an Analysis of Isolates from the Unyvero Lower Respiratory Tract Infection Trial

    Whole-genome sequencing (WGS) is now routinely performed in clinical microbiology laboratories to assess isolate relatedness. With appropriately developed analytics, the same data can be used for prediction of antimicrobial susceptibility. We assessed WGS data for identification using open-source tools and antibiotic susceptibility testing (AST) prediction using ARESdb compared to matrix-assisted laser desorption ionization-time of...

    Ines Ferreira, Stephan Beisken, Lukas Lueftinger, Thomas Weinmaier, Matthias Klein, Johannes Bacher, Robin Patel, Arndt von Haeseler, Andreas E. Posch
  • Single-Locus-Sequence-Based Typing of the <em>mgpB</em> Gene Reveals Transmission Dynamics in <span class="named-content genus-species" id="named-content-1">Mycoplasma genitalium</span>
    Epidemiology
    Single-Locus-Sequence-Based Typing of the mgpB Gene Reveals Transmission Dynamics in Mycoplasma genitalium

    Sexually transmitted infections (STIs) by Mycoplasma genitalium are a major problem worldwide, especially given their marked and rapid propensity for developing antimicrobial resistance. Since very few treatment options exist, clinicians face an important challenge in the management of the infection. In this scenario, little is known regarding the transmission...

    Miguel Fernández-Huerta, Judit Serra-Pladevall, Juliana Esperalba, Albert Moreno-Mingorance, Candela Fernández-Naval, María-Jesús Barberá, David Aparicio, Oscar Q. Pich, Tomàs Pumarola, Jorgen S. Jensen, Mateu Espasa

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