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Journal of Clinical Microbiology
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Borrelia burgdorferi

  • Comparative Analysis of the Euroimmun CXCL13 Enzyme-Linked Immunosorbent Assay and the ReaScan Lateral Flow Immunoassay for Diagnosis of Lyme Neuroborreliosis
    Bacteriology
    Comparative Analysis of the Euroimmun CXCL13 Enzyme-Linked Immunosorbent Assay and the ReaScan Lateral Flow Immunoassay for Diagnosis of Lyme Neuroborreliosis

    Diagnosis of Lyme neuroborreliosis (LNB) is challenging, as long as Borrelia-specific intrathecal antibodies are not yet detectable. The chemokine CXCL13 is elevated in the cerebrospinal fluid (CSF) of LNB patients. Here, we compared the performances of the Euroimmun CXCL13 enzyme-linked immunosorbent assay (CXCL13 ELISA) and the ReaScan CXCL13 lateral flow immunoassay (CXCL13 LFA), a rapid point-of-care test, to support the...

    Katharina Ziegler, Anca Rath, Christoph Schoerner, Renate Meyer, Thomas Bertsch, Frank Erbguth, Christian Bogdan, Jörg Steinmann, Jürgen Held
  • Free
    Expanding Access to Biospecimens for Lyme Disease Test Development
    Commentary
    Expanding Access to Biospecimens for Lyme Disease Test Development

    The laboratory diagnosis of Lyme disease relies upon serologic testing. A standard or modified two-tiered testing algorithm is used to enhance the accuracy of antibody detection. However, this approach suffers from a lack of sensitivity in early Lyme disease. Ongoing efforts to develop more sensitive antibody detection technologies and other diagnostic approaches are dependent upon the availability of quality-assured biospecimens linked...

    John L. Schmitz
  • A Multiplexed Serologic Test for Diagnosis of Lyme Disease for Point-of-Care Use
    Immunoassays
    A Multiplexed Serologic Test for Diagnosis of Lyme Disease for Point-of-Care Use

    Single multiplexed assays could replace the standard 2-tiered (STT) algorithm recommended for the laboratory diagnosis of Lyme disease if they perform with a specificity and a sensitivity superior or equal to those of the STT algorithm.

    Siddarth Arumugam, Samiksha Nayak, Taylor Williams, Francesco Serra di Santa Maria, Mariana Soares Guedes, Rodrigo Cotrim Chaves, Vincent Linder, Adriana R. Marques, Elizabeth J. Horn, Susan J. Wong, Samuel K. Sia, Maria Gomes-Solecki
  • Evaluation of the Modified Two-Tiered Testing Method for Diagnosis of Lyme Disease in Children
    Bacteriology
    Evaluation of the Modified Two-Tiered Testing Method for Diagnosis of Lyme Disease in Children

    Conventional two-tiered testing (CTTT) for Lyme disease includes a first-tier enzyme immunoassay (EIA) followed by a supplemental immunoblot, and modified two-tiered testing (MTTT) relies on two different sequential EIAs without the inclusion of an immunoblot. MTTT has shown promising results as an alternative strategy for the diagnosis of Lyme disease in adults but has not yet been evaluated in children.

    Susan C. Lipsett, John A. Branda, Lise E. Nigrovic
  • Antibodies to <span class="named-content genus-species" id="named-content-1">Borrelia turicatae</span> in Experimentally Infected Dogs Cross-React with <span class="named-content genus-species" id="named-content-2">Borrelia burgdorferi</span> Serologic Assays
    Immunoassays
    Antibodies to Borrelia turicatae in Experimentally Infected Dogs Cross-React with Borrelia burgdorferi Serologic Assays

    Tick-borne relapsing fever (TBRF) is caused by several Borrelia spp. (including Borrelia turicatae), which are primarily transmitted by Ornithodoros ticks. Relapsing fever group species are found worldwide, except for Antarctica. Approximately 500 human cases were reported between 1990 and 2011 in the United States (likely an underestimate), while...

    Jenna R. Gettings, Job E. Lopez, Aparna Krishnavajhala, Brittany A. Armstrong, Alec T. Thompson, Michael J. Yabsley
  • Genotyping and Quantifying Lyme Pathogen Strains by Deep Sequencing of the Outer Surface Protein C (<em>ospC</em>) Locus
    Bacteriology
    Genotyping and Quantifying Lyme Pathogen Strains by Deep Sequencing of the Outer Surface Protein C (ospC) Locus

    A mixed infection of a single tick or host by Lyme disease spirochetes is common and a unique challenge for the diagnosis, treatment, and surveillance of Lyme disease. Here, we describe a novel protocol for differentiating Lyme strains on the basis of deep sequencing of the hypervariable outer surface protein C locus (ospC).

    Lia Di, Zhenmao Wan, Saymon Akther, Chunxiao Ying, Amanda Larracuente, Li Li, Chong Di, Roy Nunez, D. Moses Cucura, Noel L. Goddard, Konstantino Krampis, Wei-Gang Qiu
  • Open Access
    Serological Diagnostics of Lyme Borreliosis: Comparison of Universal and <span class="named-content genus-species" id="named-content-1">Borrelia</span> Species-Specific Tests Based on Whole-Cell and Recombinant Antigens
    Immunoassays
    Serological Diagnostics of Lyme Borreliosis: Comparison of Universal and Borrelia Species-Specific Tests Based on Whole-Cell and Recombinant Antigens

    The study compares diagnostic parameters of different commercial serological kits based on three different antigen types and correlates test results with the status of the patient's Borrelia infection. In total, 8 IgM and 8 IgG kits were tested, as follows: enzyme-linked immunosorbent assay (ELISA) (Euroimmun) based on whole-cell antigen, 3 species-specific enzyme...

    Petr Kodym, Zuzana Kurzová, Dagmar Berenová, Dušan Pícha, Dita Smíšková, Lenka Moravcová, Marek Malý
  • Free
    Immunoassays
    Evaluation of Modified Two-Tiered Testing Algorithms for Lyme Disease Laboratory Diagnosis Using Well-Characterized Serum Samples

    Standard two-tiered testing (STTT) is the recommended algorithm for laboratory diagnosis of Lyme disease (LD). Several limitations are associated with STTT that include low sensitivity in the early stages of disease, as well as technical complexity and subjectivity associated with second-tier immunoblotting; therefore, modified two-tiered testing (MTTT) algorithms that utilize two sequential first-tier tests and eliminate immunoblotting...

    Adoracion Pegalajar-Jurado, Martin E. Schriefer, Ryan J. Welch, Marc R. Couturier, Tiffany MacKenzie, Rebecca J. Clark, Laura V. Ashton, Mark J. Delorey, Claudia R. Molins
  • Bacteriology
    Direct Molecular Detection and Genotyping of Borrelia burgdorferi Sensu Lato in Cerebrospinal Fluid of Children with Lyme Neuroborreliosis
    Bjørn Barstad, Hanne Quarsten, Dag Tveitnes, Sølvi Noraas, Ingvild S. Ask, Maryam Saeed, Franziskus Bosse, Grete Vigemyr, Ilka Huber, Knut Øymar
  • Open Access
    Bacteriology
    An Enzyme-Linked Immunosorbent Spot Assay Measuring Borrelia burgdorferi B31-Specific Interferon Gamma-Secreting T Cells Cannot Discriminate Active Lyme Neuroborreliosis from Past Lyme Borreliosis: a Prospective Study in the Netherlands
    T. van Gorkom, S. U. C. Sankatsing, W. Voet, D. M. Ismail, R. H. Muilwijk, M. Salomons, B. J. M. Vlaminckx, A. W. J. Bossink, D. W. Notermans, J. J. M. Bouwman, K. Kremer, S. F. T. Thijsen

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