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Carba NP

  • NitroSpeed-Carba NP Test for Rapid Detection and Differentiation between Different Classes of Carbapenemases in <em>Enterobacterales</em>
    Bacteriology
    NitroSpeed-Carba NP Test for Rapid Detection and Differentiation between Different Classes of Carbapenemases in Enterobacterales

    A biochemical test (NitroSpeed-Carba NP test) was developed to identify carbapenemase production in Enterobacterales and to discriminate between the different types of clinically significant carbapenemases (Ambler classes A, B, and D). It is based on two main features, namely, the hydrolysis by all β-lactamases, including carbapenemases of the nitrocefin substrate, and the capacity of ertapenem to prevent this hydrolysis for...

    Patrice Nordmann, Mustafa Sadek, Anthony Demord, Laurent Poirel
  • Open Access
    Systematic Comparison of Four Methods for Detection of Carbapenemase-Producing <em>Enterobacterales</em> Directly from Blood Cultures
    Bacteriology
    Systematic Comparison of Four Methods for Detection of Carbapenemase-Producing Enterobacterales Directly from Blood Cultures

    Early identification of infections caused by carbapenemase-producing Enterobacterales (CPE) can help to optimize patient treatment and improve outcome. In this study, protocols for rapid detection of carbapenemase production directly from positive blood cultures were developed applying a concentration and hemolysis step before a test for carbapenemase production was performed.

    Maria Meier, Axel Hamprecht
  • Diagnostic Accuracy of BD Phoenix CPO Detect for Carbapenemase Production in 190 <span class="named-content genus-species" id="named-content-1">Enterobacteriaceae</span> Isolates
    Bacteriology
    Diagnostic Accuracy of BD Phoenix CPO Detect for Carbapenemase Production in 190 Enterobacteriaceae Isolates

    The rapid and accurate detection of carbapenemase-producing Enterobacteriaceae (CPE) is necessary for patient management and infection control measures. We compared the performance of the BD Phoenix CPO Detect with that of a homemade Carba NP assay and a modified carbapenem inactivation method (mCIM) by challenging all 3 assays with 190 isolates of...

    Chiou Horng Ong, Lasantha Ratnayake, Michelle L. T. Ang, Raymond Tzer Pin Lin, Douglas Su Gin Chan
  • Phenotypic Detection of Carbapenemase-Producing Organisms from Clinical Isolates
    Minireview
    Phenotypic Detection of Carbapenemase-Producing Organisms from Clinical Isolates

    The rapid spread of multidrug-resistant Gram-negative organisms constitutes one of the greatest challenges to global health. While Gram-negative organisms have developed several mechanisms to avert the bactericidal effects of commonly prescribed antibiotic agents, the increasing prevalence of carbapenemase-producing organisms (CPO) is particularly concerning given the rapid spread of mobile genetic elements containing carbapenemase...

    Pranita D. Tamma, Patricia J. Simner
  • Bacteriology
    Multicenter Evaluation of the Modified Carbapenem Inactivation Method and the Carba NP for Detection of Carbapenemase-Producing Pseudomonas aeruginosa and Acinetobacter baumannii
    Patricia J. Simner, J. Kristie Johnson, William B. Brasso, Karen Anderson, David R. Lonsway, Virginia M. Pierce, April M. Bobenchik, Zabrina C. Lockett, Angella Charnot-Katsikas, Lars F. Westblade, Brian B. Yoo, Stephen G. Jenkins, Brandi M. Limbago, Sanchita Das, Darcie E. Roe-Carpenter
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