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Journal of Clinical Microbiology
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coinfection

  • Whole-Genome Sequencing of Porcine Reproductive and Respiratory Syndrome Virus from Field Clinical Samples Improves the Genomic Surveillance of the Virus
    Clinical Veterinary Microbiology
    Whole-Genome Sequencing of Porcine Reproductive and Respiratory Syndrome Virus from Field Clinical Samples Improves the Genomic Surveillance of the Virus

    Porcine reproductive and respiratory syndrome virus (PRRSV) is a major economic concern worldwide. There are currently large data sets available about the ORF5 gene of the virus, with thousands of sequences available, but little data are currently available on the full-length genome of PRRSV. We hypothesized that whole-genome sequencing (WGS) of the PRRSV genome would allow better epidemiological monitoring than ORF5 gene sequencing....

    Christian Lalonde, Chantale Provost, Carl A. Gagnon
  • Open Access
    Metagenomic Next-Generation Sequencing of the 2014 Ebola Virus Disease Outbreak in the Democratic Republic of the Congo
    Virology
    Metagenomic Next-Generation Sequencing of the 2014 Ebola Virus Disease Outbreak in the Democratic Republic of the Congo

    We applied metagenomic next-generation sequencing (mNGS) to detect Zaire Ebola virus (EBOV) and other potential pathogens from whole-blood samples from 70 patients with suspected Ebola hemorrhagic fever during a 2014 outbreak in Boende, Democratic Republic of the Congo (DRC) and correlated these findings with clinical symptoms. Twenty of 31 patients (64.5%) tested in Kinshasa, DRC, were EBOV positive by quantitative reverse...

    Tony Li, Placide Mbala-Kingebeni, Samia N. Naccache, Julien Thézé, Jerome Bouquet, Scot Federman, Sneha Somasekar, Guixia Yu, Claudia Sanchez-San Martin, Asmeeta Achari, Bradley S. Schneider, Anne W. Rimoin, Andrew Rambaut, Justus Nsio, Prime Mulembakani, Steve Ahuka-Mundeke, Jimmy Kapetshi, Oliver G. Pybus, Jean-Jacques Muyembe-Tamfum, Charles Y. Chiu
  • Mycobacteriology and Aerobic Actinomycetes
    Performance of the Abbott RealTime MTB and MTB RIF/INH Assays in a Setting of High Tuberculosis and HIV Coinfection in South Africa
    Lesley Scott, Anura David, Lara Noble, Matilda Nduna, Pedro Da Silva, Andrew Black, Francois Venter, Wendy Stevens
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