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colistin

  • Simple Phenotypic Tests To Improve Accuracy in Screening Chromosomal and Plasmid-Mediated Colistin Resistance in Gram-Negative Bacilli
    Bacteriology
    Simple Phenotypic Tests To Improve Accuracy in Screening Chromosomal and Plasmid-Mediated Colistin Resistance in Gram-Negative Bacilli

    CLSI and EUCAST recommend that only broth microdilution (BMD) should be used for routine colistin susceptibility testing; however, this technique can be difficult to perform in resource-poor settings. The purpose of this study was to evaluate the accuracy of a colistin agar spot test (COL-AS) and a colistin drop test (COL-DT) compared to BMD. COL-AS and COL-DT were assessed with a collection of 271 Gram-negative bacilli clinical...

    Fernando Pasteran, Diego Danze, Alejandra Menocal, Carla Cabrera, Ignacio Castillo, Ezequiel Albornoz, Celeste Lucero, Melina Rapoport, Paola Ceriana, Alejandra Corso
  • Evaluation of the NG-Test MCR-1 Lateral Flow Assay and EDTA-Colistin Broth Disk Elution Methods To Detect Plasmid-Mediated Colistin Resistance among Gram-Negative Bacterial Isolates
    Bacteriology
    Evaluation of the NG-Test MCR-1 Lateral Flow Assay and EDTA-Colistin Broth Disk Elution Methods To Detect Plasmid-Mediated Colistin Resistance among Gram-Negative Bacterial Isolates

    Plasmid-mediated colistin resistance (PMCR) is a global public health concern, given its ease of transmissibility. The purpose of this study was to evaluate two methods for the detection of PMCR from bacterial colonies: (i) the NG-Test MCR-1 lateral flow immunoassay (LFA; NG Biotech, Guipry, France) and (ii) the EDTA-colistin broth disk elution (EDTA-CBDE) screening test method. These methods were evaluated using a cohort of...

    Alexander J. Fenwick, Yehudit Bergman, Shawna Lewis, Rebecca Yee, Anne-Catrin Uhlemann, Nicolynn Cole, Peggy Kohner, Carly Ordak, Daniel A. Green, Audrey N. Schuetz, Romney Humphries, Patricia J. Simner
  • Phenotypic Detection of Plasmid-Mediated Colistin Resistance in <em>Enterobacteriaceae</em>
    Bacteriology
    Phenotypic Detection of Plasmid-Mediated Colistin Resistance in Enterobacteriaceae

    The aim of this work was to evaluate an easy-to-perform assay based upon inhibition of mobile colistin resistance (MCR) activity by EDTA. We included 92 nonrelated isolates of Enterobacteriaceae (74 Escherichia coli, 17 Klebsiella pneumoniae, and 1...

    Edgar Gonzales Escalante, Katherine Yauri Condor, Jose A. Di Conza, Gabriel O. Gutkind
  • Evaluation of Calcium-Enhanced Media for Colistin Susceptibility Testing by Gradient Agar Diffusion and Broth Microdilution
    Bacteriology
    Evaluation of Calcium-Enhanced Media for Colistin Susceptibility Testing by Gradient Agar Diffusion and Broth Microdilution

    Despite the increasing reliance on polymyxin antibiotics (polymyxin B and colistin) for treatment of multidrug-resistant Gram-negative infections, many clinical laboratories are unable to perform susceptibility testing due to the lack of accurate and reliable methods. Although gradient agar diffusion is commonly performed for other antimicrobials, its use for polymyxins is discouraged due to poor performance characteristics.

    ...
    Daniel A. Green, Nenad Macesic, Anne-Catrin Uhlemann, Mabel Lopez, Stephania Stump, Susan Whittier, Audrey N. Schuetz, Patricia J. Simner, Romney M. Humphries
  • Multicenter Evaluation of Colistin Broth Disk Elution and Colistin Agar Test: a Report from the Clinical and Laboratory Standards Institute
    Bacteriology
    Multicenter Evaluation of Colistin Broth Disk Elution and Colistin Agar Test: a Report from the Clinical and Laboratory Standards Institute

    Susceptibility testing of the polymyxins (colistin and polymyxin B) is challenging for clinical laboratories. The Clinical and Laboratory Standards Institute (CLSI) Antimicrobial Susceptibility Testing Subcommittee evaluated two methods to enable accurate testing of these agents. These methods were a colistin broth disk elution (CBDE) and a colistin agar test (CAT), the latter of which was evaluated using two inoculum volumes, 1 μl (CAT...

    Romney M. Humphries, Daniel A. Green, Audrey N. Schuetz, Yehudit Bergman, Shawna Lewis, Rebecca Yee, Stephania Stump, Mabel Lopez, Nenad Macesic, Anne-Catrin Uhlemann, Peggy Kohner, Nicolynn Cole, Patricia J. Simner
  • A Novel Phenotypic Method To Screen for Plasmid-Mediated Colistin Resistance among <em>Enterobacteriales</em>
    Bacteriology
    A Novel Phenotypic Method To Screen for Plasmid-Mediated Colistin Resistance among Enterobacteriales

    Plasmid-mediated colistin resistance (PMCR), a consequence of the mcr genes, is a significant public health concern given its potential to easily spread among clinical pathogens. Recently, it was discovered that MCR enzymes require zinc for activity.

    Drew T. Bell, Yehudit Bergman, Abida Q. Kazmi, Shawna Lewis, Pranita D. Tamma, Patricia J. Simner
  • Evaluation of the MicroScan Colistin Well and Gradient Diffusion Strips for Colistin Susceptibility Testing in <em>Enterobacteriaceae</em>
    Bacteriology
    Evaluation of the MicroScan Colistin Well and Gradient Diffusion Strips for Colistin Susceptibility Testing in Enterobacteriaceae

    Many laboratories are unable to perform colistin susceptibility testing. Diffusion-based antimicrobial susceptibility testing methods are not recommended, and not all laboratories have the capacity to perform broth microdilution (BMD).

    Joseph D. Lutgring, Anny Kim, Davina Campbell, Maria Karlsson, Allison C. Brown, Eileen M. Burd
  • Antimicrobial Susceptibility Testing for Polymyxins: Challenges, Issues, and Recommendations
    Minireview
    Antimicrobial Susceptibility Testing for Polymyxins: Challenges, Issues, and Recommendations

    Polymyxins, including polymyxin B and polymyxin E (colistin), are now increasingly being used worldwide to treat patients with multidrug-resistant (MDR) Gram-negative bacterial infections. This necessitates that laboratories employ an accurate and reliable method for the routine performance of polymyxin susceptibility testing.

    Fereshteh Ezadi, Abdollah Ardebili, Reza Mirnejad
  • A Resazurin Reduction-Based Assay for Rapid Detection of Polymyxin Resistance in <span class="named-content genus-species" id="named-content-1">Acinetobacter baumannii</span> and <span class="named-content genus-species" id="named-content-2">Pseudomonas aeruginosa</span>
    Bacteriology
    A Resazurin Reduction-Based Assay for Rapid Detection of Polymyxin Resistance in Acinetobacter baumannii and Pseudomonas aeruginosa

    A rapid test was developed for identification of polymyxin resistance in nonfermenting bacteria. This test detects viable cells after growth in a medium containing a defined concentration of colistin.

    Mathilde Lescat, Laurent Poirel, Camille Tinguely, Patrice Nordmann
  • A Prospective Study of <em>Acinetobacter baumannii</em> Complex Isolates and Colistin Susceptibility Monitoring by Mass Spectrometry of Microbial Membrane Glycolipids
    Bacteriology
    A Prospective Study of Acinetobacter baumannii Complex Isolates and Colistin Susceptibility Monitoring by Mass Spectrometry of Microbial Membrane Glycolipids

    Acinetobacter baumannii is a prevalent nosocomial pathogen with a high incidence of multidrug resistance. Treatment of infections due to this organism with colistin, a last-resort antibiotic of the polymyxin class, can result in the emergence of colistin-resistant strains.

    Lisa M. Leung, Christi L. McElheny, Francesca M. Gardner, Courtney E. Chandler, Sarah L. Bowler, Roberta T. Mettus, Caressa N. Spychala, Erin L. Fowler, Belita N. A. Opene, Robert A. Myers, David R. Goodlett, Yohei Doi, Robert K. Ernst

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