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Coxiella burnetii

  • High-Content Screening, a Reliable System for <span class="named-content genus-species" id="named-content-1">Coxiella burnetii</span> Isolation from Clinical Samples
    Bacteriology
    High-Content Screening, a Reliable System for Coxiella burnetii Isolation from Clinical Samples

    Q fever, caused by Coxiella burnetii, is a worldwide zoonotic disease that may cause severe forms in humans and requires a specific and prolonged antibiotic treatment. Although current serological and molecular detection tools allow a reliable diagnosis of the disease, culture of C. burnetii strains...

    Rania Francis, Maxime Mioulane, Marion Le Bideau, Marie-Charlotte Mati, Pierre-Edouard Fournier, Didier Raoult, Jacques Yaacoub Bou Khalil, Bernard La Scola
  • Comparison of Different Commercially Available Enzyme-Linked Immunosorbent Assays with Immunofluorescence Test for Detection of Phase II IgG and IgM Antibodies to <em>Coxiella burnetii</em>
    Immunoassays
    Comparison of Different Commercially Available Enzyme-Linked Immunosorbent Assays with Immunofluorescence Test for Detection of Phase II IgG and IgM Antibodies to Coxiella burnetii

    Several commercially available enzyme-linked immunosorbent assays (ELISAs) for the detection of phase II IgG or IgM antibodies against Coxiella burnetii were compared. In addition, an indirect immunofluorescence test was used as a confirmation test. In all, 70 serum samples for IgG and 43 serum samples for IgM were tested. The ELISAs showed large differences in...

    Larissa Dangel, Daniela Koempf, Silke F. Fischer
  • Introduction to Measurement of Avidity of Anti-<span class="named-content genus-species" id="named-content-1">Coxiella burnetii</span> IgG in Diagnosis of Q Fever
    Bacteriology
    Introduction to Measurement of Avidity of Anti-Coxiella burnetii IgG in Diagnosis of Q Fever

    Due to the atypical serological profile of some patients with primary Q fever infection who do not develop IgM against Coxiella burnetii, we developed an avidity test to distinguish recent or past infections. We tested 39 serum samples by immunofluorescence with conventional assay and after urea treatment from 26 patients at different stages of the disease.

    ...
    Léa Luciani, Coralie L’Ollivier, Matthieu Million, Bernard Amphoux, Sophie Edouard, Didier Raoult
  • Bacteriology
    Fluorescence In Situ Hybridization (FISH) and Peptide Nucleic Acid Probe-Based FISH for Diagnosis of Q Fever Endocarditis and Vascular Infections
    Elsa Prudent, Hubert Lepidi, Emmanouil Angelakis, Didier Raoult
  • Chlamydiology and Rickettsiology
    Contamination of Commercial PCR Master Mix with DNA from Coxiella burnetii
    Jeroen J. H. C. Tilburg, Marrigje H. Nabuurs-Franssen, Erik J. van Hannen, Alphons M. Horrevorts, Willem J. G. Melchers, Corné H. W. Klaassen
  • Bacteriology
    Interlaboratory Evaluation of Different Extraction and Real-Time PCR Methods for Detection of Coxiella burnetii DNA in Serum
    Jeroen J. H. C. Tilburg, Willem J. G. Melchers, Annika M. Pettersson, John W. A. Rossen, Mirjam H. A. Hermans, Erik J. van Hannen, Marrigje H. Nabuurs-Franssen, Maaike C. de Vries, Alphons M. Horrevorts, Corné H. W. Klaassen
  • CASE REPORTS
    Coxiella burnetii Infection of a Steller Sea Lion (Eumetopias jubatus) Found in Washington State
    Gilbert J. Kersh, Dyanna M. Lambourn, Joshua S. Self, Adrianne M. Akmajian, James B. Stanton, Timothy V. Baszler, Stephen A. Raverty, Robert F. Massung
  • Chlamydiology and Rickettsiology
    Duplex PCR Assay Simultaneously Detecting and Differentiating Bartonella quintana, B. henselae, and Coxiella burnetii in Surgical Heart Valve Specimens
    Yi-Wei Tang
  • Chlamydiology and Rickettsiology
    Comparison of PCR, Immunofluorescence Assay, and Pathogen Isolation for Diagnosis of Q Fever in Humans with Spontaneous Abortions
    V. M. Vaidya, S. V. S. Malik, Simranpreet Kaur, Satish Kumar, S. B. Barbuddhe
  • CASE REPORTS
    Chronic Q Fever in the United States
    Petros C. Karakousis, Michele Trucksis, J. Stephen Dumler

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