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Journal of Clinical Microbiology
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culture

  • Open Access
    The Isolation of <span class="named-content genus-species" id="named-content-1">Orientia tsutsugamushi</span> and <span class="named-content genus-species" id="named-content-2">Rickettsia typhi</span> from Human Blood through Mammalian Cell Culture: a Descriptive Series of 3,227 Samples and Outcomes in the Lao People’s Democratic Republic
    Chlamydiology and Rickettsiology
    The Isolation of Orientia tsutsugamushi and Rickettsia typhi from Human Blood through Mammalian Cell Culture: a Descriptive Series of 3,227 Samples and Outcomes in the Lao People’s Democratic Republic

    In the Lao People’s Democratic Republic (Laos), rickettsial infections, including scrub and murine typhus, account for a significant burden of fevers. The Mahosot Hospital Microbiology Laboratory in Vientiane, Laos, routinely performs rickettsial isolation from hospitalized patients with suspected rickettsioses using mammalian cell culture systems. We review the clinical and laboratory factors associated with successful...

    Damien K. Ming, Vanheuang Phommadeechack, Phonepasith Panyanivong, Davanh Sengdatka, Weerawat Phuklia, Vilada Chansamouth, Tamalee Roberts, Stuart D. Blacksell, Paul N. Newton, Matthew T. Robinson
  • Fast, Simple, and Cheap: the Kudoh-Ogawa Swab Method as an Alternative to the Petroff–Lowenstein-Jensen Method for Culturing of <span class="named-content genus-species" id="named-content-1">Mycobacterium tuberculosis</span>
    Mycobacteriology and Aerobic Actinomycetes
    Fast, Simple, and Cheap: the Kudoh-Ogawa Swab Method as an Alternative to the Petroff–Lowenstein-Jensen Method for Culturing of Mycobacterium tuberculosis

    The objective of this study was to compare the diagnostic yield of the Kudoh-Ogawa (K-O) swab method for the culturing of Mycobacterium tuberculosis from clinical samples with the standard Petroff–Lowenstein-Jensen (P-LJ) procedure. A total of 2,287 sputum samples and 685 extrapulmonary clinical specimens were processed with both decontamination methods and compared...

    Greta Franco-Sotomayor, Ismar A. Rivera-Olivero, Margarita Leon-Benitez, Sandra E. Uruchima-Campoverde, Greta Cardenas-Franco, Mercedes E. Perdomo-Castro, Camilo S. Cardenas-Franco, Jackeline Ortega-Vivanco, Ana S. Abad-Ruiz, Jacobus H. de Waard, Miguel Angel Garcia-Bereguiain
  • Evaluation of OMNIgene Sputum and Ethanol Reagent for Preservation of Sputum Prior to Xpert and Culture Testing in Uganda
    Bacteriology
    Evaluation of OMNIgene Sputum and Ethanol Reagent for Preservation of Sputum Prior to Xpert and Culture Testing in Uganda

    Xpert MTB/RIF (Xpert) and culture are the most reliable methods for tuberculosis diagnosis but are still poorly accessible in many low-resource countries. We aimed to assess the effects of OMNIgene Sputum (OM-S) and ethanol in preserving sputum for Xpert and OM-S for mycobacterial growth indicator tube (MGIT) testing over periods of 15 and 8 days, respectively. Sputum samples were collected from newly diagnosed smear-positive patients...

    Elisa Ardizzoni, Patrick Orikiriza, Charles Ssuuna, Dan Nyehangane, Mourad Gumsboga, Ivan Mugisha Taremwa, Esther Turyashemererwa, Juliet Mwanga-Amumpaire, Céline Langendorf, Maryline Bonnet
  • Passive Filtration, Rapid Scanning Electron Microscopy, and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for <em>Treponema</em> Culture and Identification from the Oral Cavity
    Bacteriology
    Passive Filtration, Rapid Scanning Electron Microscopy, and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Treponema Culture and Identification from the Oral Cavity

    We present here a new passive-filtration-based culture device combined with rapid identification with a new electron microscope (Hitachi TM4000) for the detection and culture of Treponema species from the human oral cavity. Of the 44 oral samples cultivated, 15 (34%) were found to be positive for Treponema using electron microscopy and were also culture positive. All were subcultured on agar plates; based on genome...

    Souad Belkacemi, Jacques Bou Khalil, Yusuke Ominami, Akiko Hisada, Anthony Fontanini, Aurelia Caputo, Anthony Levasseur, Jean-Christophe Lagier, Saber Khelaifia, Didier Raoult
  • Molecular Bacterial Load Assay Concurs with Culture on NaOH-Induced Loss of <span class="named-content genus-species" id="named-content-1">Mycobacterium tuberculosis</span> Viability
    Mycobacteriology and Aerobic Actinomycetes
    Molecular Bacterial Load Assay Concurs with Culture on NaOH-Induced Loss of Mycobacterium tuberculosis Viability

    Effective methods to detect viable Mycobacterium tuberculosis, the main causative agent of tuberculosis (TB), are urgently needed. To date, cultivation of M. tuberculosis is the gold standard, which depends on initial sample processing with N-acetyl-l-cysteine–sodium...

    Bariki Mtafya, Wilber Sabiiti, Issa Sabi, Joseph John, Emanuel Sichone, Nyanda E. Ntinginya, Stephen H. Gillespie
  • Comparative Evaluation of Enteric Bacterial Culture and a Molecular Multiplex Syndromic Panel in Children with Acute Gastroenteritis
    Bacteriology
    Comparative Evaluation of Enteric Bacterial Culture and a Molecular Multiplex Syndromic Panel in Children with Acute Gastroenteritis

    Although enteric multianalyte syndromic panels are increasingly employed, direct comparisons with traditional methods and the inclusion of host phenotype correlations are limited. Luminex xTAG gastrointestinal pathogen panel (GPP) and culture results are highly concordant.

    Thomas Kellner, Brendon Parsons, Linda Chui, Byron M. Berenger, Jianling Xie, Carey-Ann D. Burnham, Phillip I. Tarr, Bonita E. Lee, Alberto Nettel-Aguirre, Jonas Szelewicki, Otto G. Vanderkooi, Xiao-Li Pang, Nathan Zelyas, Stephen B. Freedman
    and on behalf of the Alberta Provincial Pediatric EnTeric Infection TEam (APPETITE)
  • Human Bacterial Repertoire of the Urinary Tract: a Potential Paradigm Shift
    Minireview
    Human Bacterial Repertoire of the Urinary Tract: a Potential Paradigm Shift

    The aim of this article is to review the human repertoire of bacteria in urine already described by culture and metagenomic techniques and published in the literature. Our study led us to compare this repertoire with other available human repertoires.

    Aurélie Morand, Florent Cornu, Jean-Charles Dufour, Michel Tsimaratos, Jean-Christophe Lagier, Didier Raoult
  • Free
    The Brief Case: Retropharyngeal Abscess in a 14-Year-Old Caused by <span class="named-content genus-species" id="named-content-1">Fusobacterium necrophorum</span>
    The Brief Case
    The Brief Case: Retropharyngeal Abscess in a 14-Year-Old Caused by Fusobacterium necrophorum
    Tam T. Van, Samia N. Naccache, Jennifer Dien Bard
  • Free
    Closing the Brief Case: Retropharyngeal Abscess in a 14-Year-Old Caused by <span class="named-content genus-species" id="named-content-1">Fusobacterium necrophorum</span>
    The Brief Case
    Closing the Brief Case: Retropharyngeal Abscess in a 14-Year-Old Caused by Fusobacterium necrophorum
    Tam T. Van, Samia N. Naccache, Jennifer Dien Bard
  • Free
    Sonication versus Tissue Sampling for Diagnosis of Prosthetic Joint and Other Orthopedic Device-Related Infections
    Bacteriology
    Sonication versus Tissue Sampling for Diagnosis of Prosthetic Joint and Other Orthopedic Device-Related Infections

    Current guidelines recommend collection of multiple tissue samples for diagnosis of prosthetic joint infections (PJI). Sonication of explanted devices has been proposed as a potentially simpler alternative; however, reported microbiological yield varies.

    Maria Dudareva, Lucinda Barrett, Mel Figtree, Matthew Scarborough, Masanori Watanabe, Robert Newnham, Rachael Wallis, Sarah Oakley, Ben Kendrick, David Stubbs, Martin A. McNally, Philip Bejon, Bridget A. Atkins, Adrian Taylor, Andrew J. Brent

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