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Journal of Clinical Microbiology
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detection

  • Use of a Multiplex PCR Assay To Assess the Presence of <span class="named-content genus-species" id="named-content-1">Treponema pallidum</span> in Mucocutaneous Ulcerations in Patients with Suspected Syphilis
    Bacteriology
    Use of a Multiplex PCR Assay To Assess the Presence of Treponema pallidum in Mucocutaneous Ulcerations in Patients with Suspected Syphilis

    We evaluated the utility of the commercial Allplex genital ulcer real-time PCR multiplex assay for detecting Treponema pallidum (TPA), herpes simplex virus 1 (HSV-1) and 2 (HSV-2), and Chlamydia trachomatis serovar L (lymphogranuloma venereum [LGV]) DNA in mucosal and genital ulcers in the context...

    P. A. Grange, A. Jary, C. Isnard, S. Burrel, D. Boutolleau, A. Touati, C. Bébéar, J. Saule, P. Martinet, J.-L. Robert, D. Moulene, A. Vermersch-Langlin, N. Benhaddou, M. Janier, N. Dupin
  • Changing from Clinician-Collected to Self-Collected Throat Swabs for Oropharyngeal Gonorrhea and Chlamydia Screening among Men Who Have Sex with Men
    Bacteriology
    Changing from Clinician-Collected to Self-Collected Throat Swabs for Oropharyngeal Gonorrhea and Chlamydia Screening among Men Who Have Sex with Men

    The coronavirus disease 2019 (COVID-19) pandemic has led many clinics to move from clinician-collected to self-collected oropharyngeal swabs for the detection of sexually transmitted infections (STIs). Before this change, however, self-collection was used primarily for genital and anorectal infections, with only limited studies on the performance of self-collection of oropharyngeal swabs for oropharyngeal STI detection. The Melbourne...

    Eric P. F. Chow, Catriona S. Bradshaw, Deborah A. Williamson, Shauna Hall, Marcus Y. Chen, Tiffany R. Phillips, Ria Fortune, Kate Maddaford, Christopher K. Fairley
  • High-Content Screening, a Reliable System for <span class="named-content genus-species" id="named-content-1">Coxiella burnetii</span> Isolation from Clinical Samples
    Bacteriology
    High-Content Screening, a Reliable System for Coxiella burnetii Isolation from Clinical Samples

    Q fever, caused by Coxiella burnetii, is a worldwide zoonotic disease that may cause severe forms in humans and requires a specific and prolonged antibiotic treatment. Although current serological and molecular detection tools allow a reliable diagnosis of the disease, culture of C. burnetii strains...

    Rania Francis, Maxime Mioulane, Marion Le Bideau, Marie-Charlotte Mati, Pierre-Edouard Fournier, Didier Raoult, Jacques Yaacoub Bou Khalil, Bernard La Scola
  • Open Access
    Evaluation of the ResistancePlus MG FleXible Assay for Detection of Wild-Type and 23S rRNA-Mutated <span class="named-content genus-species" id="named-content-1">Mycoplasma genitalium</span> Strains
    Bacteriology
    Evaluation of the ResistancePlus MG FleXible Assay for Detection of Wild-Type and 23S rRNA-Mutated Mycoplasma genitalium Strains

    Antibiotic resistance in Mycoplasma genitalium is rising globally, and resistance-guided diagnostics can facilitate targeted and timely treatment. The ResistancePlus MG FleXible (RPMG Flex) assay for the detection of M. genitalium and macrolide resistance-mediating mutations (MRMM) was evaluated for...

    Suhella Tulsiani Drud, Peter Njuguna, Samantha Ebeyan, Simon Erskine, Mette Holm, Susanne Cramer Johansson, Lit Yeen Tan, Jorgen Skov Jensen
  • Single-Cell-Based Digital PCR Detection and Association of Shiga Toxin-Producing <span class="named-content genus-species" id="named-content-1">Escherichia coli</span> Serogroups and Major Virulence Genes
    Clinical Veterinary Microbiology
    Single-Cell-Based Digital PCR Detection and Association of Shiga Toxin-Producing Escherichia coli Serogroups and Major Virulence Genes

    Escherichia coli serogroups O157, O26, O45, O103, O111, O121, and O145, when carrying major virulence genes, the Shiga toxin genes stx1 and stx2 and the intimin gene eae, are important foodborne pathogens. They are referred to as the “top 7” Shiga toxin-producing...

    Xuming Liu, Lance Noll, Xiaorong Shi, Elizabeth Porter, Yin Wang, Colin Stoy, Nanyan Lu, T. G. Nagaraja, Gary Anderson, Jianfa Bai
  • Clinical Evaluation of Three Commercial PCR Assays for the Detection of Macrolide Resistance in <span class="named-content genus-species" id="named-content-1">Mycoplasma genitalium</span>
    Bacteriology
    Clinical Evaluation of Three Commercial PCR Assays for the Detection of Macrolide Resistance in Mycoplasma genitalium

    As macrolide resistance in Mycoplasma genitalium is increasing worldwide, macrolide resistance-associated mutations should be assessed in M. genitalium-positive specimens. New commercial kits are available for detection of macrolide resistance concurrently with...

    Chloé Le Roy, Cécile Bébéar, Sabine Pereyre
  • A Cost-Effective Method for Identifying <em>Enterobacterales</em> with OXA-181
    Bacteriology
    A Cost-Effective Method for Identifying Enterobacterales with OXA-181

    OXA-181 is the second most common global OXA-48-like carbapenemase and is endemic in the Indian subcontinent. Molecular studies have shown that Enterobacterales with OXA-181 are often introduced into regions of nonendemicity. Distinguishing OXA-181 from other OXA-48-like enzymes often requires sequencing, which is rather expensive and time-consuming.

    Gisele Peirano, Yasufumi Matsumura, Diego Nobrega, Johann D. D. Pitout
  • Open Access
    Development and Multicentric Validation of a Lateral Flow Immunoassay for Rapid Detection of MCR-1-Producing <em>Enterobacteriaceae</em>
    Immunoassays
    Development and Multicentric Validation of a Lateral Flow Immunoassay for Rapid Detection of MCR-1-Producing Enterobacteriaceae

    Colistin has become a last-resort antibiotic for the treatment of infections caused by highly drug-resistant Gram-negative bacteria. Moreover, it has been widely used in the livestock sector.

    Hervé Volland, Laurent Dortet, Sandrine Bernabeu, Hervé Boutal, Marisa Haenni, Jean-Yves Madec, Frédéric Robin, Racha Beyrouthy, Thierry Naas, Stéphanie Simon
  • Evaluation of the New Multiplex Immunochromatographic O.K.N.V. <em>K</em>-SeT Assay for Rapid Detection of OXA-48-like, KPC, NDM, and VIM Carbapenemases
    Letter to the Editor
    Evaluation of the New Multiplex Immunochromatographic O.K.N.V. K-SeT Assay for Rapid Detection of OXA-48-like, KPC, NDM, and VIM Carbapenemases
    C. Kolenda, R. Benoit, A. Carricajo, R. Bonnet, O. Dauwalder, F. Laurent
  • Clinical Veterinary Microbiology
    Modified Protein Misfolding Cyclic Amplification Overcomes Real-Time Quaking-Induced Conversion Assay Inhibitors in Deer Saliva To Detect Chronic Wasting Disease Prions
    Kristen A. Davenport, Clare E. Hoover, Nathaniel D. Denkers, Candace K. Mathiason, Edward A. Hoover

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