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diarrhea

  • Ultrasensitive <span class="named-content genus-species" id="named-content-1">Clostridioides difficile</span> Toxin Testing for Higher Diagnostic Accuracy
    Minireview
    Ultrasensitive Clostridioides difficile Toxin Testing for Higher Diagnostic Accuracy

    Currently available diagnostic tests for Clostridioides difficile infection (CDI) lack specificity or sensitivity, which has led to guideline recommendations for multistep testing algorithms. Ultrasensitive assays for detection of C. difficile toxins provide measurements of disease-specific markers...

    Johanna Sandlund, Kerrie Davies, Mark H. Wilcox
  • Evaluation of Anatomically Designed Flocked Rectal Swabs for Use with the BioFire FilmArray Gastrointestinal Panel for Detection of Enteric Pathogens in Children Admitted to Hospital with Severe Gastroenteritis
    Bacteriology
    Evaluation of Anatomically Designed Flocked Rectal Swabs for Use with the BioFire FilmArray Gastrointestinal Panel for Detection of Enteric Pathogens in Children Admitted to Hospital with Severe Gastroenteritis

    Diagnosing diarrheal disease is difficult in part due to challenges in obtaining and transporting a bulk stool specimen, particularly in resource-limited settings. We compared the performance of flocked rectal swabs to that of traditional bulk stool samples for enteric pathogen detection using the BioFire FilmArray gastrointestinal panel in children admitted to four hospitals in Botswana with community onset severe gastroenteritis....

    Celia R. Walker, Kwana Lechiile, Margaret Mokomane, Andrew P. Steenhoff, Tonya Arscott-Mills, Jeffrey M. Pernica, David M. Goldfarb
  • Pigment Visibility on Rectal Swabs Used To Detect Enteropathogens: a Prospective Cohort Study
    Epidemiology
    Pigment Visibility on Rectal Swabs Used To Detect Enteropathogens: a Prospective Cohort Study

    Data are lacking regarding the impact of visible pigment on rectal swab diagnostic accuracy. We describe the test characteristics of rectal swabs with and without pigment in children with gastroenteritis.

    Jianling Xie, Gillian A. M. Tarr, Samina Ali, Linda Chui, Xiao-Li Pang, Bonita E. Lee, Otto G. Vanderkooi, Phillip I. Tarr, Ran Zhuo, Brendon Parsons, Byron M. Berenger, Kelly Kim, Stephen B. Freedman
    and on behalf of the Alberta Provincial Pediatric EnTeric Infection TEam (APPETITE) and Pediatric Emergency Research Canada (PERC)
  • Culture of Rectal Swab Specimens for Enteric Bacterial Pathogens Decreases Time to Test Result While Preserving Assay Sensitivity Compared to Bulk Fecal Specimens
    Bacteriology
    Culture of Rectal Swab Specimens for Enteric Bacterial Pathogens Decreases Time to Test Result While Preserving Assay Sensitivity Compared to Bulk Fecal Specimens

    Diarrheal illness is a major cause of morbidity and mortality throughout the world, yet the etiologic agent of many cases of gastrointestinal illness remains unspecified, often due to the lack of convenient, timely, and sensitive diagnostic testing. Although bulk fecal specimens remain the recommended specimen type for enteric culture, rectal swabs may be an option preferred by clinicians and patients due to the convenience and timing...

    Sophonie Jean, Melanie L. Yarbrough, Neil W. Anderson, Carey-Ann D. Burnham
  • Virulence-Related Genes and Coenteropathogens Associated with Clinical Outcomes of Enteropathogenic <em>Escherichia coli</em> Infections in Children from the Brazilian Semiarid Region: a Case-Control Study of Diarrhea
    Bacteriology
    Virulence-Related Genes and Coenteropathogens Associated with Clinical Outcomes of Enteropathogenic Escherichia coli Infections in Children from the Brazilian Semiarid Region: a Case-Control Study of Diarrhea

    Enteropathogenic Escherichia coli (EPEC) is a major cause of diarrhea in children from developing countries and presents high genetic variability. We aimed to characterize the EPEC virulence-related gene (VRG) distribution and copathogens associated with diarrhea and nutrition-related outcomes in children from the low-income Brazilian semiarid region.

    ...
    Ana Karolina S. Santos, Pedro Henrique Q. S. de Medeiros, Mariana D. Bona, Mara M. G. Prata, Marília S. M. G. Amaral, Herlice N. Veras, Rafaela C. Pankov, Samilly A. Ribeiro, Paloma A. Cavalcante, Thiago M. Freitas, Rafhaella D. G. Gondim, Daniel M. N. de Oliveira, Natália K. F. M. Melo, Alexandre Havt, Aldo A. M. Lima
  • Epidemiology
    Rapid Molecular Detection of Gastrointestinal Pathogens and Its Role in Antimicrobial Stewardship
    Şiran Keske, Burak Zabun, Kahraman Aksoy, Füsun Can, Erhan Palaoğlu, Önder Ergönül
  • Bacteriology
    Low Yield of FilmArray GI Panel in Hospitalized Patients with Diarrhea: an Opportunity for Diagnostic Stewardship Intervention
    Matthew M. Hitchcock, Carlos A. Gomez, Niaz Banaei
  • Open Access
    Bacteriology
    Direct Detection of Shigella in Stool Specimens by Use of a Metagenomic Approach
    Jie Liu, Mathieu Almeida, Furqan Kabir, Sadia Shakoor, Shahida Qureshi, Anita Zaidi, Shan Li, Boubou Tamboura, Samba O. Sow, Inacio Mandomando, Pedro L. Alonso, Thandavarayan Ramamurthy, Dipika Sur, Karen Kotloff, James Nataro, Myron M. Levine, O. Colin Stine, Eric Houpt
  • Bacteriology
    A Gastrointestinal PCR Panel Improves Clinical Management and Lowers Health Care Costs
    Stacy G. Beal, Elizabeth E. Tremblay, Steven Toffel, Lymaries Velez, Kenneth H. Rand
  • Bacteriology
    Multisite Evaluation of the BD Max Extended Enteric Bacterial Panel for Detection of Yersinia enterocolitica, Enterotoxigenic Escherichia coli, Vibrio, and Plesiomonas shigelloides from Stool Specimens
    Patricia J. Simner, Margret Oethinger, Kathleen A. Stellrecht, Dylan R. Pillai, Ram Yogev, Helene Leblond, Joel Mortensen

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