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DNA extraction

  • Open Access
    Optimizing DNA Extraction Methods for Nanopore Sequencing of <span class="named-content genus-species" id="named-content-1">Neisseria gonorrhoeae</span> Directly from Urine Samples
    Bacteriology
    Optimizing DNA Extraction Methods for Nanopore Sequencing of Neisseria gonorrhoeae Directly from Urine Samples

    Empirical gonorrhea treatment at initial diagnosis reduces onward transmission. However, increasing resistance to multiple antibiotics may necessitate waiting for culture-based diagnostics to select an effective treatment. There is a need for same-day culture-free diagnostics that identify infection and detect antimicrobial resistance. We investigated if Nanopore sequencing can detect sufficient...

    Teresa L. Street, Leanne Barker, Nicholas D. Sanderson, James Kavanagh, Sarah Hoosdally, Kevin Cole, Robert Newnham, Mathyruban Selvaratnam, Monique Andersson, Martin J. Llewelyn, Justin O’Grady, Derrick W. Crook, David W. Eyre
    and the GonFast Investigators Group
  • Open Access
    Clinical Veterinary Microbiology
    Optimization of Brucella abortus Protocols for Downstream Molecular Applications
    Noah Hull, Jonathan Miller, David Berry, William Laegreid, Ashley Smith, Callie Klinghagen, Brant Schumaker
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